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            北京大学生命科学学院上目录导师简介(46人)


            北京大学生命科学学院上目录导师简介( 北京大学生命科学学院上目录导师简介(46 人) 目录导师简介
            安成才, 教授,博士生导师 .......................................................................................................................... 3 白书农, 教授,博士生导师 .......................................................................................................................... 4 蔡 宏, 教授,博士生导师 ............................................................................................................................ 5 柴 真, 教授,博士生导师 ............................................................................................................................ 6 昌增益, 教授,博士生导师 .......................................................................................................................... 7 陈建国, 教授,博士生导师 ........................................................................................................................ 10 邓宏魁, 教授,博士生导师 ........................................................................................................................ 12 邓兴旺, 教授,博士生导师 ........................................................................................................................ 13 顾红雅, 教授,博士生导师 ........................................................................................................................ 14 顾 军, 教授,博士生导师 .......................................................................................................................... 15 郭红卫, 教授,博士生导师 ........................................................................................................................ 17 纪建国, 教授,博士生导师 ........................................................................................................................ 18 蒋争凡, 研究员,博士生导师 .................................................................................................................... 19 金长文, 教授,博士生导师 ........................................................................................................................ 20 孔道春, 教授,博士生导师 ........................................................................................................................ 22 李 毅, 教授,博士生导师 .......................................................................................................................... 23 林 硕, 教授,博士生导师(Cheung Kong Visiting Scholar) ............................................................. 24 刘 东, 研究员,博士生导师 ...................................................................................................................... 25 刘 磊, 研究员,博士生导师 ...................................................................................................................... 27 龙漫远, 教授,博士生导师 ........................................................................................................................ 28 罗述金, 研究员,博士生导师 .................................................................................................................... 30 吕 植, 教授,博士生导师 .......................................................................................................................... 31 秦咏梅, 教授,博士生导师 ........................................................................................................................ 32 瞿礼嘉, 教授,博士生导师 ........................................................................................................................ 34 饶广远, 教授,博士生导师 ........................................................................................................................ 35 饶 毅, 讲席教授,博士生导师 .................................................................................................................. 36 苏都莫日根, 教授,博士生导师 ................................................................................................................ 38 苏晓东, 教授,博士生导师 ........................................................................................................................ 39 陶乐天, 研究员,博士生导师 .................................................................................................................... 40 王世强, 教授,博士生导师 ........................................................................................................................ 40 王忆平, 教授,博士生导师 ........................................................................................................................ 42 魏丽萍, 教授,博士生导师 ........................................................................................................................ 43 魏文胜, 研究员,博士生导师 .................................................................................................................... 44 夏 斌, 教授,博士生导师 .......................................................................................................................... 45 谢灿, 研究员,博士生导师 ........................................................................................................................ 45 许智宏, 院士,教授,博士生导师 ............................................................................................................ 47 翟中和, 院士,教授,博士生导师 ............................................................................................................ 48 张 博, 教授,博士生导师 .......................................................................................................................... 49 张传茂, 教授,博士生导师 ........................................................................................................................ 51 郑晓峰, 教授,博士生导师 ........................................................................................................................ 52 朱玉贤, 教授,博士生导师 ........................................................................................................................ 55
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            朱作言, 院士,教授,博士生导师 ............................................................................................................ 56 范六民, 教授,博士生导师 ........................................................................................................................ 57 滕俊琳, 副教授,博士生导师 .................................................................................................................... 59 王戎疆, 副教授,博士生导师 .................................................................................................................... 60 张 研, 副教授,博士生导师 ...................................................................................................................... 61

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            安成才, 教授, 安成才 教授,博士生导师

            Email: chcaian@pku.edu.cn TEL: TEL: 86 - 10 - 6275-2405 Fax: Fax:86 - 10 - 6275-9070 科研领域描述 植物代谢调控及生物药物:(1)植物代谢调控的分子机理;(2)生物药物基因工程。 主要研究兴趣: 主要研究兴趣: 1. 植物抗病防卫反应的调控机理: 植物对病原物信号的传递及其抗病防卫反应的激发与解除激 活的分子机理 2. 天花粉蛋白导向药物 ScFv-TCS 及其癌细胞定向识别与选择性杀伤机制 3. 油料植物脂肪酸合成代谢的分子机理及其高产油料作物的分子育种技术研究 4. 控藻生物的遗传工程改良及其在富营养化水体修复中的应用技术研究 代表性论文 ? Li Xu, Ye Wang, Lijiang Wang, Yin Gao, Chengcai An* , TYchi, a novel chitinase with RNA N-glycosidase and anti-tumor activities , Frontiers in Bioscience , 2008 , 13: 3127-3135 ? Liu Xinyao, Kong Shuanglei, Shi Miao, Fu Liwen, Gao Yin and An Chengcai* , Genomic Analysis of Freshwater Cyanophage Pf-WMP3 Infecting Cyanobacterium Phormidium foveolarum: The Conserved Elements for a Phage , Microbial Ecology , 2008 , 56:671-680 ? Li Xu, Lijiang Wang, Tao Liu, Wanqiang Qian, Yin Gao, Chengcai An , Triton, a novel family of miniature inverted-repeat transposable elements (MITEs) in Trichosanthes kirilowii Maximowicz and its effect on gene regulation , BBRC , 2007 , 364: 668-674 ? Tao Liu, Jia-yu Gu, Chen-jia Xu, Yin Gao, Cheng-cai An* , Overproduction of OsSLRL2 alters the development of transgenic Arabidopsis plants , BBRC , 2007 , 358: 983 –989 ? Wanqiang Qian, Guihong Tan, Shanping He, Xinyue Zhang, Xu Zhang, Yin Gao, Hongxia Liu and Chengcai An* , Identification of a new AT-rich-element binding factor PsATF1 and its combined effect with PsGBF on the activation of PsCHS1 promoter , Frontiers in Bioscience , 2007 , Vol. 12: 1670-1679 ? Xinyao Liu, Miao Shi, Shuanglei Kong, Yin Gao, Chengcai An* , Cyanophage Pf-WMP4, a T7-like phage infecting the freshwater cyanobacterium Phormidium foveolarum: Complete genomesequence and DNA translocation , Virology , 2007 , 366: 28–39 ? Xin-Yue Zhang, Ye Wu, Jin-Yuan Yan, Yin Gao, Ye Wang, Shuang-Li Mi, Cheng-Cai An* , Y55 and D78 are crucial amino acid residues of a new IgE epitope on trichosanthin , BBRC , 2006 , 343(4):1251-1256 ? Liu Xinyao, Shi Miao, Liao Yonghong, Gao Yin, Zhang Zhongkai, Wen Donghui, Wu Weizhong and An Chengcai* , Isolation of an amoebae capable of grazing Anabaena flos-aquae from Dianchi Lake and its identification by SSU rRNA gene , Microbial Ecology , 2006 , Vol. 51: 315-325

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            Shuang-li Mi, Cheng-cai An*, Ye Wang, Ji-yuan Chen, Nan-ying Che, Yin Gao and Zhang-liang Chen , Trichomislin, a novel ribosome-inactivating protein, induces apoptosis that involves mitochondria and caspase-3 , Archives of Biochemistry and Biophysics , 2005 , 434(2): 258-265 ? Guihong Tan, Yin Gao, Miao Shi, Xinyue Zhang, Shanping He, Zhangliang Chen and Chengcai An* , SiteFinding-PCR: a simple and efficient PCR method for chromosome walking , Nucleic Acids Research , 2005 , Vol. 33, No. 13 e122 ? Yan Yuanxin, An Chengcai*, Li Li, Gu Jiayu, Tan Guihong and Chen Zhangliang , T-linker Specific Ligation PCR (T-linker-PCR): An advanced PCR technique for chromosome walking or for isolation of tagged DNA ends , Nucleic Acids Research , 2003 , Vol. 31(12): 1-7 e68 ? Chunyang Zhang, Chengcai An*, Rongying Wang, Yixuan Gong, Hui Ma, Dieyan Chen & Zhangliang Chen , Capillary Electrophoresis and Circular Dichroism Study of Trichosanthin and Its Mutants , Talanta , 2002 , 57: 467-473 ==============================================

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            白书农, 教授, 白书农 教授,博士生导师

            TEL: TEL: 86 - 10 - 6275-1444 Fax: Fax:86 - 10 - 6275-1526 科研领域描述 本实验室主要兴趣在于: 1 以雄蕊为对象的植物器官形成调控机制 2 植物发育事件相关基因表达在染色质水平调控 植物器官形成主要指来自顶端分生组织的一团未分化细胞如何形成具有特定形态与功能的三维 结构,是植物发育中的一个重要过程。我们利用雄蕊为对象,从分析导致黄瓜雌花雄蕊发育停滞的 分子机制、和发育早期不同阶段的水稻雄蕊基于表达谱两个角度入手,尝试解析雄蕊早期发育这一 复杂的多细胞结构形态建成过程中关键的发育事件及其在分子水平的调控机制。这将不仅帮助我们 了解雄蕊形成的机制,还可以帮助我们了解多细胞结构形态建成过程中的一些基本规律。 在我们前期的工作中,发现基因表达的染色质水平调控在植物发育事件中具有重要的作用。因 此,我们面前利用拟南芥根表皮细胞分化过程中模式形成这一发育事件,探讨发育相关基因表达在 染色质水平的调控机制,并希望利用在此研究中所积累的经验,帮助我们理解雄蕊早期发育的调控 机制。 代表性论文 ? Duan, QH et al. , Stamen development in Arabidopsis is arrested by organ-specific overexpression of a cucumber ethylene synthesis gene CsACO2 , Planta , 2008 , 228 (4): 537-543 ? Lu, XC et al. , Photoperiodic responses of various morphogenetic events after panicle initiation in late japonica rice (Oryza sativa L. vs. japonica). , Plant Biology , 2008 , 10: 388-397 ? Lu and Gong et al. , Molecular analysis of early rice stamen development using organ-specific gene expression profiling , Plant Mol. Biol. , 2006 , 61: 845-861

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            Xu CR et al. , Histone acetylation affects expression of cellular patterning genes in the Arabidopsis root epidermis , PNAS , 2005 , 102: 14469-14474 ? Bai SL et al. , Developmental analyses reveal early arrests of the spore-bearing parts of reproductive organs in unisexual flowers of cucumber (Cucumis sativus L.). , Planta , 2004 , 220 (2): 230-240 ? Hao YJ Wang DH et al. , DNA damage in early primordial anther is closely correlated with the stamen arrest in female flower of cucumber (Cucumis sativus L.) , Planta , 2003 , 217(6): 888-95 ====================================================

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            教授, 蔡 宏, 教授,博士生导师

            Email: hcai@pku.edu.cn Fax: Fax:86 - 10 - 6275-4427 TEL: TEL: 86 - 10 - 6275-6077 科研领域描述 本实验室主要兴趣在于: 1. 多价 DNA 疫苗的分子机理。 2. 基因治疗的机制研究。 3. 植物反应器的研究。 结核病是一种人畜共患病,严重威胁着人类的健康。传统的 BCG 疫苗的保护效率不一致。DNA 疫苗可诱导体液和细胞免疫应答,而结核杆菌和布氏杆菌都是胞内寄生菌,DNA 疫苗是未来非常具 有开发前景的新型疫苗。实验室通过动物实验筛选优良的免疫主导性抗原,并通过 DNA 免疫的手段 测定其保护效率及在动物保护中的作用机制。目前为止,建立了一套可靠的核酸疫苗免疫、检测评 价体系,比较了各种多价不同组合及其分别与不同佐剂保或包裹剂混合免疫的保护效力,探讨了多 种提高疫苗效力和持续期的策略和可行方法,并研究了各种方法在激发机体免疫机制上的差异和途 径。 除了探讨 DNA 疫苗在预防方面的作用,我们通过 DNA 疫苗结合传统化学药物的方法对受感染的 小鼠及牛进行基因治疗,进而探讨在感染后,DNA 疫苗对机体内免疫反应类型的改变状况。 为进一步推进疫苗的实用性,我们构建了马铃薯来源的植物疫苗,动物可口服该疫苗而无须打 针。通过动物实验,植物疫苗在小鼠体内诱导了体液免疫(尤其是粘膜免疫应答)和细胞应答反应。 代表性论文 ? Hu Xi-Dan, Chen Su-Ting, Yu Da-Hai, Li Shu-Xia, Cai Hong , Immunotherapy with combined DNA vaccines is an effective treatment for M. bovis infection in cattle. , VACCINE , 2009 , 27:1317-1322 ? Hu Xi-Dan, Yu Da-Hai, Chen Su-Ting, Li Shu-Xia, Cai Hong , A Combined DNA Vaccine Provides Protective Immunity Against Mycobacterium bovis and Brucella abortus in Cattle. , DNA AND CELL BIOLOGY , 2009 , 28(4):191-199 ? Li Min, Yu Da-Hai,Cai Hong , The Synthetic Antimicrobial Peptide KLKL5KLK Enhances Cai the Protection and Efficacy of the Combined DNA Vaccine against Mycobacterium tuberculosis. , DNA AND CELL BIOLOGY , 2008 , 27(8):405-413

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            Yu Da-Hai,Hu Xi-Dan,Cai Hong , Efficient tuberculosis treatment in mice using Cai chemotherapy and immunotherapy with the combined DNA vaccine encoding Ag85B, MPT-64 and MPT-83. , GENE THERAPY , 2008 , 15:652–659 ? Yu Da-Hai, Li Min, Hu Xi-Dan, Cai Hong , A Combined DNA Vaccine Enhances Protective Immunity against Mycobacterium tuberculosis and Brucella abortus in the Presence of an IL-12 Expression Vector. , VACCINE , 2007 , 25(9):6744-6754 ? Yu Da-Hai, Cai Hong* Hu Xi-Dan, Li Min , A Combined DNA Vaccine Encoding BCSP31, Hong**, SOD and L7/L12 Confers High Protection against Brucella abortus 2308 by Inducing Specific CTL Responses. , DNA AND CELL BIOLOGY , 2007 , 26 (6): 435-443 ? Cai Hong*, Yu Da-Hai, Hu Xi-Dan, Li Shu-Xia, and Zhu Yu-Xian. , A Combined DNA Vaccine-Prime, BCG-Boost Strategy Results in Better Protection against Mycobacterium. bovis Challenge. , DNA AND CELL BIOLOGY , 2006 , 25 (8): 438-447 ? Cai Hong* Hong**, Yu Da-Hai, Tian Xia, Zhu Yu-Xian. , Co-administration of interleukin 2 plasmid DNA with combined DNA vaccines enhance significantly the protective efficacy against Mycobacterium Tuberculosis. , DNA AND CELL BIOLOGY , 2005 , 24 (10): 605-613 ? Cai Hong* Hong**, Hu Xidan, Yu Da-Hai, Li Shu-Xia, Zhu Yu-Xian. , Combined DNA vaccine encapsulated in microspheres enhanced protection efficacy against Mycobacterium tuberculosis infection of mice. , VACCINE , 2005 , 23 (32): 4167-4174 ? Cai Hong* Tian Xia, Hu Xidan, Li Shu-Xia, Yu Da-Hai, Zhu Yu-Xian. , Combined DNA Hong**, vaccines formulated either in DDA or in saline protect cattle from Mycobacterium bovis infection. , VACCINE , 2005 , 23 (30): 3887-3895 ? Cai Hong Tian Xia, Hu Xi-Dan, Zhuang Yu-Hui, Zhu Yu-Xian* , Combined DNA Vaccines Hong, Formulated in DDA Enhance Protective Immunity against Tuberculosis. , DNA AND CELL BIOLOGY , 2004 , 23 (7): 450-456 =================================================

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            教授, 柴 真, 教授,博士生导师

            Fax: Fax:86 - 10 - 6275-1526 TEL: TEL: 86 - 10 - 6275-7830 科研领域描述 本实验室主要兴趣在于: 1. 脑损伤和修复过程中神经元钙稳态的调控机制。 2. 神经元和胶质细胞低氧损伤或适应的细胞机制。 3. 细胞因子及其信号转导途径在体温调节中的作用。 代表性论文 ? Tang AH, Chai Z and Wang SQ* , Dark rearing alters the short-term synaptic plasticity in visual cortex. , Neurosci Lett , 2007 , 206: 799-806 ? Zhou C, Ye HH, Wang SQ, Chai Z , Interleukin-1β regulation of N-type Ca2+ channels Z* in cortical neurons. , Neurosci Lett , 2006 , 403: 181-185.

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            Zhou C, Tai C, Ye HH, Ren X, Chen JG, Wang, SQ, Chai Z , Interleukin-1 β Z* downregulates the L-Type Ca2+ channel activity by depressing the expression of channel protein in cortical neurons. , J Cell Physiol , 2006 , 206: 799-806. ? Wang TF, Zhou C, Tang AH, Wang SQ, Chai Z , Cellular mechanism for spontaneous Z* calcium oscillations in astrocytes. , Acta Pharmacol Sinica , 2006 , 27: 861-868 ? Fu Y, Zhang GQ, Hao XM, Wu CH, Chai Z Wang SQ* , Temperature dependence and Z, thermodynamic properties of Ca sparks in rat cardiomyocytes. , Biophys J , 2005 , 89: 2533-2541. ? Alheim K, Chai Z Fantuzzi G, Hasanvan H, Di Santo E, Ghezzi P, Dinarello CA, Bartfai Z, T* , Hyperresponsive febrile reactions to IL-1 β in IL-1 β deficient mice; comparison with cytokine mRNA and protein levels. , Proc Natl Acad Sci USA , 1997 , 94: 2681-2686. ? Chai Z, Gatti S, Toniatti C, Poli V, Bartfai T* , Interleukin (IL)-6 gene expression in the central nervous system is necessary for fever response to lipopolysaccharide or IL-1β: a study on IL-6-deficient mice. , J Exp Med , 1996 , 183: 311-316 ? Loudkvist J, Chai Z Teheranian R, Hasanvan H, Bartfai T, Jenck F, Widmer U, Moreau Z, JL , A non peptidic corticotropin releasing factor receptor antagonist attenuates fever and exhibits anxiolytic-like activity. , Eur J Pharmacol , 1996 , 309: 195-200. ? Chai Z Alheim K, Lundkvist J, Gatti S, Bartfai T* , Subchromic glucocorticoid Z, pretreatment reversibly attenuates IL-1β induced fever in rats; IL-6 mRNA is elevated while IL-1α and IL-1β mRNAs are suppressed, in the CNS. , Cytokine , 1996 , 8: 227-237. ? Malinowsky D, Chai Z, Bristulf J, Simoncsits A, Bartfai T* , The type I interleukin-1 receptor mediates fever in rat as shown by interleukin-1 receptor subtype selective ligands. , Neurosci lett , 1995 , 201: 33-36. ==============================================

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            昌增益, 教授, 昌增益 教授,博士生导师

            生命科学学院副院长, 北京大学跨院系蛋白质科学中心 主任 Email: changzy@pku.edu.cn Fax: Fax:86 - 10 - 62751526 TEL: TEL: 86 - 10 - 62758822 科研领域描述 本实验室主要兴趣在于: 1. 生 物 的 胁 迫 响 应 , 包 括 衰 老 和 休 眠 现 象 的 分 子 机 制 (Stress responses of living organisms: including the molecular mechanism of aging and dormancy) 2. 生物体内蛋白质-蛋白质相互作用的发生情况、机制、及生物学意义 (Mechanism and biological significance of protein-protein interactions) 任何生命形式都不可避免地要经历像高温、 极端 pH 值、 活性氧等有毒物质的存在等胁迫条件。 在这样的条件下,生物体内的大分子,如 DNA、RNA、蛋白质等由非共价弱相互作用维持的空间结构 都将受到影响。这其中可能以蛋白质分子受到的威胁最大,蛋白质分子的去折叠往往会导致聚集的 发生,而这将意味着蛋白质分子功能的丧失。过去的研究表明,各种生物的基因组都编码了一类被
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            称为分子伴侣(或热休克蛋白、或胁迫蛋白等)的蛋白质分子。这些蛋白质的角色就是保护生物体 内那些容易发生去折叠和聚集的蛋白质。 “保镖” 这些 蛋白质, 参与了对体内其它蛋白质分子的 “生、 老、病、死”等过程。我们关注的一些问题包括:胁迫蛋白质是如何在生物体内发挥其功能的?胁 迫蛋白质分子的结构如何对胁迫条件发生瞬时响应而导致生物学活性顺时性提高的?部分生命个体 以休眠方式应对极端胁迫条件的分子机制是什么?与此相关的衰老现象和休眠现象的分子机制是什 么?等等。 蛋白质在生物体内发挥作用是通过与其它分子发生相互作用而完成的。大量的蛋白质分子自 身或相互之间也发生着大量的相互作用。自身的相互作用,形成同源寡聚体,不同蛋白质分子之间 的相互作用即形成异源寡聚体。尽管过去的体外或体内研究已经揭示了大量特异的蛋白质之间的相 互作用,但大量发生于生物体内的动态的或弱的相互作用目前还难以鉴定和深入研究。我们关注的 一些问题包括:很多通过体外研究被鉴定为以单亚基形式存在的蛋白质,在体内是否能够形成同源 寡聚体?能够在活细胞条件下鉴定出与特异蛋白质发生相互作用的蛋白质?这样的以同源或异源寡 聚形式发生的蛋白质-蛋白质相互作用如何完成特定的生物学功能?等等。 代表性论文 ? Zengyi Chang , The CUSBEA Program: Twenty Years Later , IUBMB Life , 2009 , 61:(6)555-565 (feature article) ? Zengyi Chang , Posttranslational modulation of Biological Activities of Molecular Chaperones , Science in China Series C: Life Sciences , 2009 , 52(6):515-520. , Invited Critical Review , [Full Text] ? Yunbiao Wang, Anastasia N. Ezemaduka, Yan Tang and Zengyi Chang , Understanding the Mechanism of the Dormant Dauer Formation of C. elegans: From Genetics to Biochemistry , IUBMB Life , 2009 , 61(6):607-612. , invited critical review ? FENG Yongjun, ZHANG Meng, HU Mingxi, ZHENG Jie, JIAO Wangwang & CHANG Zengyi , Disassembly Intermediates of RbsD Protein remain Oligomeric despite the loss of an intact secondary structure , Sci. China Ser. C-Life Sciences , 2009 , in press ? Jiang, J.S., Zhang, X. F., Chen, Y., Wu, Y., Zhou, Z. H., Chang, Z. Sui, S.F. , Z., Activation of DegP Chaperone-Protease via Formation of Large Cage-like Oligomers upon Binding to Substrate Proteins , Proc. Natl. Acad. Sci. USA , 2008 , 105(33):11939-11944. , 通讯作者(corresponding author) ? Jiao, W., Hong, W., Li, P., Sun, S., Ma, J., Qian, M., Hu, M., and Chang, Z. , The Dramatically Increased Chaperone Activity of Small Heat Shock Protein IbpB is Retained for an Extended Period of Time after the Stress Condition is Removed , Biochem. J. , 2008 , 15;410(1):63-70. , [Abstract] ? Liu, C., Mao, K., Zhang, M., Sun, Z., Hong, W., Li, C., Peng, B., and Chang, Z. , The SH3-Like Domain Switches Its Interaction Partners to Modulate the Repression Activity of Mycobacterial Iron-Dependent Transcription Regulator (IdeR) in Response to Metal Ion Fluctuations , J. Biol. Chem. , 2008 , Vol. 283, Issue 4, 2439-2453 , [Abstract] , [Full Text] ? Wu, Y., Hong, W., Zhang, L., and Chang, Z. , Conserved Amphiphilic Feature Is Essential for Periplasmic Chaperone HdeA to Support Acid Resistance in Enteric Bacteria , Biochem. J. , 2008 , 412:389-397. , [Abstract] ? 马静, 葛熙、昌增益 , 蛋白质功能研究:历史、现状和将来 , 生命科学 , 2007 , 19(3): 昌增益 294-300 , 应邀综述

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            Feng, Y., Jiao, W., Fu, X., and Chang, Z , Stepwise Disassembly and Apparent Z. Non-stepwise Reassembly for the Oligomeric RbsD Protein , Protein Science , 2006 , 15(6):1441-1448. Fu, X., and Chang, Z , Identification of bis-ANS binding sites in Mycobacterium tuberculosis small heat shock protein Hsp16.3: Evidences for a two-step substrate-binding mechanism , Biochem. Biophys. Res. Commun. , 2006 , 349:169-171. Hong, W., Jiao, W., Hu, J., Zhang, J., Liu, C., Fu, X., Shen, D., Xia, B., and Chang, Z. , Periplasmic protein HdeA exhibits chaperone-like activity exclusively within stomach pH range by transforming into disordered conformation , J Biol Chem. , 2005 , Vol. 280, Issue 29, 27029-27034 , [Abstract] , [Full Text] Jiao, Qian, M., Li, P., Zhao, L., and Chang, Z. , The essential role of the flexible W., termini in the temperature-responsiveness of the oligomeric state and chaperone-like activity for the polydisperse small heat shock protein IbpB from Escherichia coli , J Mol Biol. , 2005 , Volume 347, Issue 4, 8 April 2005, Pages 871-884 , [Full Text] Fu, X., Zhang, H., Zhang, X., Cao, Y., Jiao, W., Liu, C., Song, Y., Abulimiti, A., and Chang, Z. , A dual role for the N-terminal region of Mycobacterium tuberculosis Hsp16.3 in self-oligomerization and binding denaturing substrate proteins , J Biol Chem. , 2005 , Vol. 280, Issue 8, 6337-6348 , [Abstract] , [Full Text] Zhang, H. Fu, X. Jiao, W., Zhang, X., Liu, C., and Chang, Z , The association of Z. small heat shock protein Hsp16.3 with the plasma membrane of Mycobacterium tuberculosis: dissociation of oligomers is a prerequisite , Biochem Biophys Res Commun., , 2005 , 330:1055-1061. Jiao, W., Li, P., Zhang, J., Zhang, H., Chang, Z , Small Heat Shock Proteins Function Z. in the Insoluble Protein Complex , Biochem Biophys Res Commun., , 2005 , 335(1):227-231. Fu, X. and Chang ,Z. , Phylogenetic and biochemical studies reveal a potential evolutionary origin of animal small heat shock proteins from bacterial class A , J. Mol. Evol., , 2005 , 62:257-266. Zhang, X.,Fu, X., Zhang, H., Liu, C. Jiao, W., and Chang, Z. , Chaperone-like Activity of β-Casein , Interna. J. Biochem. Cell Biol., , 2005 , 37:1232-1240. Fu, X., Zhang, X., and Chang, Z , 4'-dianilino-1,1'-binaphthyl-5,5'-sulfonate Z. (bis-ANS), a novel molecule having chaperone-like activity , Biochem Biophys Res Commun., , 2005 , 329:1087-1093. Liu Y., Fu, X., Shen, J., Zhang, H., Hong, W., and Chang, Z. , Periplasmic proteins of Escherichia coli are highly resistant to aggregation: reappraisal for roles of molecular chaperones in periplasm , Biochem Biophys Res Commun. , 2004 , Volume 316, Issue 3, 9 April 2004 , [Full Text] 昌增益, 昌增益 焦旺旺 , 细胞内一种耗能蛋白质降解途径的发现: 2004 年诺贝尔化学奖工作介绍 , 生物物理学报 , 2004 , 第 20 卷第 6 期,第 421-425 页。 , 应邀综述 Liu, C. He., Y. and Chang Z , Truncated hemoglobin of Mycobacterium tuberculosis: Z. The oligomeric state change and the interaction with membrane components , Biochem Biophys Res Commun. , 2004 , 316:1163-1172. Liu Y., Fu, X., Shen, J., Zhang, H., Hong, W., and Chang, Z , Periplasmic proteins Z. of Escherichia coli are highly resistant to aggregation: A reappraisal for roles
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            of molecular chaperones in periplasm , Biochem Biophys Res Commun. , 2004 , 316(3):795-801. ? Abulimiti, A., Fu, X., Gu., L., Feng, X., and Chang, Z. , Mycobacterium tuberculosis Hsp16.3 nonamers are assembled and re-assembled via trimer and hexamer intermediates , J Mol Biol. , 2003 , Volume 326, Issue 4, 28 February 2003, Pages 1013-1023 , [Full Text] ? Fu, X. Liu, C., Liu, Y., Feng, X., Gu, L., Chen, X., and Chang, Z , Small Heat Shock Z. Protein Hsp16.3 Modulates Its Chaperone Activity by Adjusting the Rate of Oligomeric Dissociation , Biochem Biophys Res Commun. , 2003 , 310(2):412-420. ? Abulimiti, A., Qiu, X., Chen, J., Liu, Y., and Chang, Z. , Reversible methionine sulfoxidation of Mycobacterium tuberculosis small heat shock protein Hsp16.3 and its possible role in scavenging oxidants , Biochem. Biophys. Res. Commun., , 2003 , 305(1):87-93. ? Gu, L., Abulimiti, A., Li, W., and Chang, Z. , Monodisperse Hsp16.3 nonamer exhibits dynamic dissociation and reassociation, with the nonamer dissociation prerequisite for chaperone-like activity , J Mol Biol. , 2002 , Volume 319, Issue 2, 31 May 2002, Pages 517-526 , [Full Text] ? Dai, H., Mao, Q., Yang, H., and Chang, Z. , Probing the Roles of the Only Universally Conserved Leucine Residue in the Oligomerization and Chaperone-like Activity of Mycobacterium tuberculosis Small Heat Shock Protein Hsp16.3 , J. Protein Chem., (new name: Protein J.) , 2000 , 19(4):319-326. ? Yang, H., Huang, S., Dai, H., Gong, Y., Zheng, C., and Chang, Z. , The Mycobacterium tuberculosis small heat shock protein Hsp16.3 exposes hydrophobic surfaces at mild conditions: conformational flexibility and molecular chaperone activity , Protein Sci. , 1999 , 8(1):174-179 , [Full Text] ? Chang, Z. Primm, T.P., Jakana, J., Lee, I.H., Chiu, W., Gilb ert, H.F., and Quiocho, Z., F.A. , Mycobacterium tuberculosis 16-kDa antigen (Hsp16.3) functions as an oligomeric structure in vitro to suppress thermal aggregation , J Biol Chem. , 1996 , 271(12):7218-23 , [Abstract] , [Full Text] ? Chang, Z Choudhary, A. Lathigra, R. and Quiocho, F.A. , The Immunodominant 38-kDa Z., Lipoprotein Antigen of Mycobacterium tuberculosis Is a Phosphate-binding Protein , J. Biol. Chem., , 1994 , 269(3):1956-1958. ? Chang, Z Nygaard, P., Chinault, A. C., and Kellems, R. , Deduced Amino Acid Sequence Z., of Escherichia coli Adenosine Deaminase Reveals Evolutionarily Conserved Amino Acid Residues: Implications for Catalytic Function , Biochemistry , 1991 , 30(8):2273-2280. =======================================

            陈建国, 教授, 陈建国 教授,博士生导师

            Fax:86 - 10 - 6275-4427 ax: TEL: TEL: 86 - 10 - 6275-5786 科研领域描述
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            本实验室主要兴趣在于: 1. 细胞骨架的分子生物学 2. 神经系统的发育及细胞分化 3. 病毒与宿主细胞的相互作用 代表性论文 ? Chen ZHOU, Chao TAI, Hai-Hong YE, Xi REN, Jianguo CHEN Shi-Qiang WANG, AND Zhen CHEN, Chai , Interleukin-1b Downregulates the L-Type Ca2t Channel Activity by Depressing the Expression of Channel Protein in Cortical Neurons. , J. of Cellular Physiology , 2006 , 206(3): 799-806. ? Feng Qian, Yingying Liu, Albert C H Yu and Jianguo Chen , Baculovirus surface display of SARS Coronavirus (SARS-CoV) spike protein and immunogenicity of the displayed protein in mice model , DNA and Cell Biology. , 2006 , 25: 668-673. ? Huihui Li, Yan Guo, Junlin Teng, Mingxiao Ding, Albert Cheung Hoi Yu and Jianguo Chen , 14-3-3gamma affects dynamics and integrity of glial filaments by binding to phosphorylated GFAP , J. of Cell Science. , 2006 , 119: 4452-4461 ? Qian Feng, Huan Yu, Yingying Liu, Chengqiang He, Jingsong Hu, Huachun Sang, Neizheng Ding, Mingxiao Ding, Yin-Wan Wendy Fungc, Lok-Ting Lauc, Albert Cheung-Hoi Yuc, Jianguo Chen , Genome comparison of a novel foot-and-mouth disease virus with other Jianguo FMDV strains , BBRC. , 2004 , 323: 254-263. ? Keqiang Fan, Ping Wei, Qian Feng, Sidi Chen, Changkang Huang, Liang Ma, Bing Lai, Jianfeng Pei, Ying Liu, Jianguo Chen and Luhua Lai , Biosynthesis, Purification, Chen, and Substrate Specificity of Severe Acute Respiratory Syndrome Coronavirus 3C-like Proteinase. , J. of Biological Chemistry. , 2004 , 279(3): 1637–1642 ? Qian Feng, Xi Chen, Ou Ma, Yingying Liu, Mingxiao Ding, ,Richard A. Collins, Lung-Sang Ko, Jun Xing, Lok-Ting Lau, , Albert Cheung Hoi. Yu and Jianguo Chen , Serotype Chen. and VP1 Gene Sequence of a Foot-and-Mouth Disease Virus from Hong Kong , BBRC. , 2003 , 302: 715-721. ? Xi Chen, Qian Feng, Zhiyin Wu, Yingying Liu, Kun Huang, Rui Shi, Sidi Chen, Wen Lu, Mingxiao Ding, Richard A. Collins, Albert Cheung-Hoi Yu and Jianguo Chen , RNA-dependant RNA Polymerase Gene Sequence from Foot-and Mouth Disease Virus in Hong Kong. , BBRC. , 2003 , 308: 899-905 ? Chen XQ, Chen JG Zhang Y, Hsiao WW and Yu AC , 14-3-3gamma Is upregulated by in JG, vitro ischemia and binds to protein kinase Raf in primary cultures of astrocytes , Glia. , 2003 , 42: 315-24. ? Chengrong Lu, Yong Li, Yanlin Zhao, Guichun Xing, Fei Tang, Qingming Wang,Yuhui Sun, Handong Wei, Xiaoming Yang, Chutse Wu, Jianguo Chen Kun-liang Guan, Chenggang Zhang, Chen, Huipeng Chen, Fuchu He. , Intracrine hepatopoietin potentiates AP-1 activity through JAB1 independent of MAPK pathway. , The FASEB J. , 2002 , 16: 90-92 ? Junlin Teng, Yosuke Takei, Akihiro Harada, Takao Nakata, Jianguo Chen and Nobutaka Hirokawa , Synergistic effects of MAP2 and MAP1B knockout in neuronal migration, dendritic outgrowth, and microtubule organization. , J. Cell Biology. , 2001 , 155: 65-76.

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            Song huaceng, Zhonghua Lu, Yulong Li, Binggen Ru, Wenqing Wang, and Jianguo Chen , Phosohorylation of GSK-3 beta in intact cells influences the stability of microtubules , Neuroscience Leter. , 2001 , 312: 141-144. ? Chen, J J., Nakata, T., Zhang, Z. and Hirokawa, N , The C-terminal tail domain of neurofilament protein-H (NF-H) forms the crossbridges and regulates neurofilament bundle formation , J. of Cell Science. , 2000 , 113: 3861-3869 ? Terunaga Nakagawa, Jianguo Chen Zhizeng Zhang, Yoshimitsu Kanai, and Nobutaka Chen, Hirokawa , Two Distinct Function of the Carboxyl-terminal Tail Domain of NF-M upon Neurofilament Assembly: Cross-bridge Formation and Longitudinal Elongation of Filaments , J. Cell Biol., , 1995 , 129:411-429. ? Chen, J Kanai, Y., Cowan, N. J. and Hirokawa, N. , Projection domains of MAP2 and J., tau determine spacings between microtubules in dendrites and axons , Nature. , 1992 , 360, 674 - 677. ? Kanai, Y., Chen, J and Hirokawa, N. , Microtubule bundling by tau proteins in vivo: J. analysis of functional domains , EMBO J. , 1992 , 11, 3953 - 3961 ==================================================

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            邓宏魁, 教授, 邓宏魁 教授,博士生导师

            Fax: Fax:86 - 10 - 6275-6474 TEL: TEL: 86 - 10 - 6275-6954 科研领域描述 本实验室主要兴趣在于: 1. 体细胞重编程 2. 人胚胎干细胞定向分化 3. 通过遗传修饰的手段建立疾病动物模型 代表性论文 ? Zhang P, Li J, Wang C, Tan Z, Liu T, Chen L, Yong J, Jiang W, Sun X, Ding M, Deng H, , Short-term BMP-4 treatment initiates mesoderm induction in human embryonic stem cells. , Blood , 2008 , 111(4): 1933-41. ? Jiang W, Bai Z, Zhang D, Shi Y, Yong J, Chen S, Ding M, Deng H , Differentiation H, of Mouse Nuclear Transfer Embryonic Stem Cells into Functional Pancreatic Beta Cells. , Diabetologia , 2008 , in press ? Qin H, Yu T, Qing T, Liu Y, Zhao Y, Cai J, Li J, Song Z, Qu X, Zhou P, Wu J, Ding M, Deng H , Regulation of apoptosis and differentiation by p53 in human embryonic H, stem cells. , J Biol Chem , 2007 , 282(8): 5842-52. ? Cai J, Zhao Y, Ye F, Liu Y, Song Zhi, Qin H, Meng S, Chen Y, Zhou R, Song X, Guo Y, Ding M, Deng H , Directed differentiation of human embryonic stem cells into H, functional hepatic cells. , Hepatology , 2007 , 45(5): 1229-1239. ? Ren L, Gao G, Zhao D, Ding M, Luo J, Deng H , Developmental stage related patterns H, of codon usage and genomic GC content: searching for evolutionary fingerprint by models of stem cell differentiation. , Genome Biol , 2007 , 12;8(3):R35

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            Zheng A, Yuan F, Li Y, Zhu F, Hou P, Li J, Song X, Ding M, Deng H , Claudin-6 and H, Claudin-9 Function as Additional co-Receptors for Hepatitis C Virus. , J Virol , 2007 , 81(22): 12465-71. ? Qin H, Yu T, Qing T, Liu Y, Zhao Y, Cai J, Li J, Song Z, Qu X, Zhou P, Wu J, Ding M, Deng H , Regulation of apoptosis and differentiation by p53 in human embryonic H, stem cells. , J Biol Chem , 2007 , 282(8): 5842-52. ? Wang C, Faloon P, Tan Z, Lv Y. Zhang P, Deng H Xiong J, , Mouse Lycat Controls the H, Development of Hematopoietic and Endothelial Lineages during in vitro ES Cell Differentiation. , Blood , 2007 , 110(10): 3601-9. ? Qing T, Liu H, Wei W, Ye X, Shen W, Zhang D, Song Z, Yang W, Ding M, Deng H , Mature oocytes derived from purified mouse fetal germ cells. , Human Reproduction , 2007 , 134(2):223-31. ? Shi Y, Hou L, Tang F, Jiang W, Wang P, Ding M, Deng H , Inducing Embryonic Stem H, Cells to Differentiate into Pancreatic Beta Cells by a Novel Three-Step Approach with Activin A and All-Trans Retinoic Acid. , Stem Cells , 2005 , 23(5): 656-62. 52. ? Deng H Unutmaz D, Kewalramani V, Littman D , Expression cloning of novel coreceptors H, utilized by the simion and human immunodeficency viruses , Nature , 1997 , 388:296-300. ? Deng H Liu R, Ellmeier W, Choe S, Untmaz D, Burkhart M, Marazio P, Marmon S, Sutton H, R., Hill C, Davis C, Peiper S, Schall T, Littman D, Landau N , Identification of a major co-receptor for primary isolates HIV-1 , Nature , 1996 , 381:661-666. ====================================================

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            邓兴旺, 教授, 邓兴旺 教授,博士生导师

            TEL: TEL: 86 - 10 - 6275-1895 Fax: Fax:86 - 10 - 6275-1895 科研领域描述 本实验室主要兴趣在于: 1. 赤霉素信号与逆境及脱落酸信号之间的交互作用。 赤霉素合成或信号转导中某些关键基因的突变导致作物矮化,可增强作物的抗逆能力并提高 作物的产量,故被称为绿色革命基因。对赤霉素信号转导分子机理的解析既具有重要的理论意义又 有广阔的应用前景。我们以拟南芥和水稻作为模式植物,通过基因组学、分子遗传学、生物化学等 方法和手段对植物发育过程中赤霉素信号转导过程进行深入和系统的研究,以期发现赤霉素信号转 导过程中的新的组成分子,研究它们与已知蛋白之间交互作用的分子网络。 逆境是影响植物生长发育的主要环境因子。逆境信号转导中脱落酸起非常重要的作用。我们以 拟南芥和水稻作为模式植物进行逆境及脱落酸信号转导途径中关键因子的发掘,研究它们的生物学 功能及其与农艺形状的关系。另外一个研究重点是,用遗传学、生物化学和分子遗传学方法揭示植 物发育及逆境中赤霉素与脱落酸信号之间的交互作用。 代表性论文 ? Zhou J, Wang X, Jiao Y, Qin Y, Liu X, He K, Chen C, Ma L, Wang J, Xiong L, Zhang Y, Q, Fan LM, Deng XW , Rice employs largely organ-specific programs in regulation XW.
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            of genome expression in response to drought and high-salinity stresses. , Plant Mol Biol , 2007 , 63: 591-608. ? Fan LM* (corresponding author), Feng X, Wang Y, Deng XW (corresponding author). , XW* Gibberellin signal transduction in rice. , J Integr Plant Biol , 2007 , 49: 731-741. , Review ? Bai SL, Zhong X, Ma L, Zheng W, Fan LM, Wei N, Deng XW , A simple and reliable assay XW. for detecting specific nucleotide sequence variations in plants using optical thin-film biosensor chips. , Plant Journal , 2007 , 49(2): 354-66 ? Chen H, Shen Y, Tang X, Yu L, Wang J, Guo L, Zhang Y, Zhang H, Feng S, Strickland E, Zheng N, Deng XW , Arabidopsis CULLIN4 Forms an E3 Ubiquitin Ligase with RBX1 XW. and the CDD Complex in Mediating Light Control of Development. , Plant Cell. , 2006 , ? Shen Y, Feng S, Ma L, Lin R, Qu LJ, Chen Z, Wang H, Deng XW , Arabidopsis FHY1 Protein XW. Stability Is Regulated by Light via Phytochrome A and 26S Proteasome. , Plant Physiol. , 2005 , 139(3):1234-43. ? Ma L, Chen C, Liu X, Jiao Y, Su N, Li L, Wang X, Cao M, Sun N, Zhang X, Bao J, Li J, Pedersen S, Bolund L, Zhao H, Yuan L, Wong GK, Wang J, Deng XW Wang J. , A XW, microarray analysis of the rice transcriptome and its comparison to Arabidopsis. , Genome Res. , 2005 , 15(9):1274-83. ? Ma L, Sun N, Liu X, Jiao Y, Zhao H, Deng XW , Organ-specific expression of Arabidopsis XW. genome during development. , Plant Physiol. , 2005 , 138(1):80-91. ? Ning Su, James A. Sullivan and Xing Wang Deng , Modulation of F1 hybrid stature Deng. without altering parent plants through trans-activated expression of a mutated rice GAI homologue. , Plant Biotechnology Journal. , 2005 , 3:157-164 ? Gao Y, Li J, Strickland E, Hua S, Zhao H, Chen Z, Qu L, Deng XW , An arabidopsis promoter microarray and its initial usage in the identification of HY5 binding targets in vitro , Plant Mol Biol. , 2004 , 54(5):683-99. ? Ma, L.G., Zhao, H.Y., and Deng, X.W , Analysis of the mutational effects of the COP/DET/FUS loci on genome expression profiles reveals their overlapping yet not identical roles in regulating Arabidopsis seedling development , Development , 2003 , 130: 969-981 ? Suhua Feng, Ligeng Ma, Xiping Wang, Daoxin Xie, S. P. Dinesh-Kumar, Ning Wei, Xing Wang Deng , The COP9 Signalosome Interacts Physically with SCFCOI1 and Modulates Jasmonate Responses , Plant Cell , 2003 , 15 (5): 1083-94 ==================================================

            顾红雅, 教授, 顾红雅 教授,博士生导师

            Fax: Fax:86 - 10 - 6275-1841 TEL: TEL: 86 - 10 - 6275-1847 科研领域描述 本实验室主要兴趣在于: 1. 植物系统进化学及植物分子生物学的研究 2. 拟南芥野生居群的遗传多样性及分子适应性的研究
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            3. 植物基因家族的功能和进化研究 研究对编码 CHS、水稻胰蛋白酶抑制剂等基因家族的分子进化,发现多数植物的 CHS 基因可被 分为两类,一类进化速率较慢,另一类进化速率较快且受到正选择的作用。CHS 基因在裸子植物中 的拷贝数较少,而在被子植物中拷贝数变化很大。水稻中 Bowman-Birk 型胰蛋白酶抑制剂(RBBI) 10 个成员的系统树及编码基因的 5’和 3’非编码区的序列分析发现有两个结构域受到了正向选择。 对长城两侧的六种植物的居群遗传分化进行了分析, 发现两侧的植物亚居群发生了显著的遗传分化, 证明了长城在一定程度上阻断了两侧植物的基因流,或因其两侧的环境的微小差异造成了植物居群 的变异。收集了全国约 10 个省份的 30 多个野生拟南芥居群,对其遗传多样性及分子系统树的研究 表明,有一些居群可能很早就分布于中国,并在中国迅速扩张;对不同居群在逆境下的表达谱分析 则发现明显的差异,进一步深入的研究正在进行中。 代表性论文 ? He F, Kang D, Ren Y, Qu L-J, Zhen Y and Gu H , Genetic diversity of the natural H, populations of Arabidopsis thaliana in China. , Heredity, , 2007 , 99: 423-431. ? Yang X, Li J, Pei M, Gu H Chen·Z, Qu L-J, , Over-expression of a flower-specific H, transcription factor gene AtMYB24 causes aberrant anther development. , Plant Cell Rep., , 2007 , 26: 219–228. ? Li J, Li X, Guo L, Lu F, Feng X, He K, Wei L, Chen Z, Qu L-J and Gu H , A subgroup H, of MYB transcription factor genes undergoes highly conserved alternative splicing in Arabidopsis and rice. , Journal of Experimental Botany , 2006 , 57(6): 1263-1273 ? Li J, Yang X, Wang Y, Li X, Gao Z, Pei M, Chen Z, Qu L-J and Gu H , Two groups of H, MYB transcription factors share a motif which enhances trans-activation activity. , Biochemical and Biophysical Research Communications , 2006 , 341(4): 1155-1163. ? Guo L, Wang Z, Lin H, Cui W, Chen J, Liu M, Chen Z, Qu L-J, Gu H , Expression and H, functional analysis of rice plasma-membrane intrinsic protein gene family. , Cell Research , 2006 , 16(3): 277-286. ? Su H, Qu L-J, He K, Zhang Z, Wang J, Chen Z and Gu H , The Great Wall of China: H, a physical barrier to gene flow? , Heredity , 2003 , 90: 212-219 ? Zhang W, Qu L-J, Gao W, Gu H, Chen Z, , Studies on the origin and evolution of H tetraploid wheats-based on internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA. , Theoretical and Applied Genetics , 2002 , 104: 1099-1106 ==================================================

            教授, 顾 军, 教授,博士生导师

            TEL: TEL: 86 - 10 - 6275-6174 TEL: TEL: 86 - 10 - 6275-9940 科研领域描述 本实验室主要兴趣在于: 1 炎症及炎症相关疾病(心血管,癌症等)的发生发展机制. 2 基因工程及基于生物技术的疾病治疗方法研究. 3 炎症是机体的免疫反应。炎症失控和过度可以损伤机体甚至产生致死,同时炎症又和许多疾病的 发生发展关系密切。我们的研究兴趣主要集中在先天免疫的信号通路是如何参与炎症调控的,这些 信号通路是如何与炎症相关疾病发生关联的。哪些信号分子和基因可以作为药物靶点。研究和发现
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            这些靶点以及作用这些靶点的内源分子是我们另一研究领域。同时我们在利用现代生物技术发展治 疗方法上进行相关研究. 代表性论文 ? Wang J, Wu S, Jin X, Li M, Chen S, Teeling JL, Perry VH, Gu J , Retinoic Acid-Inducible Gene-I Mediates Late Phase Induction of TNF-{alpha} by Lipopolysaccharide , J Immunol , 2008 , Jun 15;180(12):8011-9 ? Yufei Zou, Yahui Chen, Yongqiang Jiang Jin Gao and Jun Gu , Targeting Matrix Metalloproteinases and endothelial cells with a fusion peptide against tumor , Cancer Res , 2007 , 67:7295-7300 ? He J, Chen S. and Gu J , Identification and characterization of Harobin, a novel fibrino(geno)lytic serine protease from a sea snake (Lapemis hardwickii) , FEBS Lett , 2007 , 581(16):2965-73 ? Ziqiang Yang, Wei Cheng, Lixin Hong, Shengcai Lin, Jiahuai Han, Huamin Zhou and Jun Gu , Adenine nucleotide (ADP/ATP) translocase 3 participates in the tumor necrosis factor-induced apoptosis of MCF-7 cells , Molecular Biology of the Cell , 2007 , 18: 4681-89. ? Jiang YQ, Wang HR, Li HP, Hao HJ, ZhangYL and Gu J , Targeting of hepatoma cell and suppression of tumor growth by a novel 12mer peptide fused to superantigen TSST-1 , J. Mol Med , 2006 , 12, 81-87 ? . Wei JX, Sun ZG, Chen QL and Gu J , Serum deprivation induced apoptosis in macrophage J. is mediated by autocrine secretion of type I IFNs , Apoptosis , 2006 , 11(4):545-54 ? Jing Fu, Ziqiang Yang,Jinxue Wei,Jiahuai Han and Jun Gu , NP60 nuclear protein Gu. regulates p38 MAPK activity. , J Cell Sci. , 2006 , 119(Pt 1):115-23. ? Fu WX, Wei JX and Gu J , MEF2C mediates the activation induced cell death (AICD) J. of macrophage. , Cell Res. , 2006 , 16, 559-565 ? He JY, Deng J, Zheng J and Gu J , A synergistic effect on the production of S-adenosyl-l-methionine in Pichia pastoris by knocking in of S-adenosyl-l-methionine synthase and knocking out of cystathionine-beta synthase , J Biotechnol , 2006 , 126(4), 519-527. ? Xue-Dong Wang, Jian-Guo Tang, Xiao-lei Xie, Ji-Chun Yang, Shuai Li, Jian-Guo Ji and Jun Gu , A comprehensive study of optimal conditions for naked plasmid DNA transfer into skeletal muscle by electroporation. , J Gene Med , 2005 , 7:1235-1245 ? Liu BN, Tang JG, Ji JG, and Gu J , The expression of functional human parathyroid homone in a gene therapy model for osteoporosis , Cell Tissue Res , 2004 , 317, 57-63 ? Kegang Shang, Xiaoke wang, and Jun Gu , Differentiation of Embryonic Stem Cell to Astrocytes Visualized by Green Fluoresent Protein Fuchou Tang , Cellular and Molecular Neurobiology , 2002 , 22: 95-101 ? J.Sun, MT.LI, J.Han and J.Gu. , Sensitization of differentiated PC12 cells to J.Gu apoptpsis by presenilin-2mediated by p38 , Biochem. Biophy. Res. Com. , 2001 , 287; 536-541, 2001 ? J.Gu et al.in International Human Genome Consortium , Initial sequencing and analysis of the human genome , Nature , 2001 , 409:860-921 ? W.Zhu, J.S.Downey, J.GU F.D.Padova, H.Gram and J.Han , Regulation of TNF expression J.GU, by multiple MAP kinase pathway , J. Immunology , 2000 , 164:6349-6358
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            郭红卫, 教授, 郭红卫 教授,博士生导师

            Fax: Fax:86 - 10 - 6275-1526 TEL: TEL: 86 - 10 - 6276-7823 科研领域描述 主要研究方向: 1. 拟南芥中乙烯信号转导及植物激素间相互作用的分子机制 2. 植物衰老的激素调控机理 3. 小 RNA 分子和 RNA 降解的机理研究 4. 植物激素的功能基因组和蛋白质组学研究 本研究组主要课题是研究植物激素乙烯信号转导的分子机制,以及在植物发育、衰老和胁迫反 应中乙烯与其它激素和信号途径的相互作用。我们利用拟南芥作为模式系统,研究乙烯在生长发育 过程中信号转导的分子机制,发现了 EIN3/EIL1 转录因子在乙烯信号途径中的关键作用;阐明了 EIN3/EIL 蛋白的调控机理,建立了乙烯反应中由泛素/蛋白酶体介导的蛋白降解模型。另外,发现 了一个外切核酸酶 EIN5 在乙烯反应途径中的重要调节作用,揭示了 EIN5 的作用是通过促进相关基 因的 mRNA 降解来拮抗 EIN3 蛋白降解,从而建立了乙烯反应中小 RNA 分子介导的 RNA 降解模型。同 时,我们还发现乙烯和许多植物信号途径(包括光、胁迫条件、病原侵染及其它植物激素)在不同 的植物发育及抗性过程中存在复杂的相互作用。因此我们将致力于研究乙烯作用过程的分子步骤及 其调控机制,阐明在植物发育、衰老和胁迫抗性中乙烯和其他信号途径的相互作用机理。同时,我 们将结合功能基因组学和蛋白质组学来探索系统研究植物信号转导的新方法。 代表性论文 ? Li, H. and Guo, H , Molecular basis of the ethylene signaling and response pathway in Arabidopsis , Journal of Plant Growth Regulation , 2007 , 26:89-210 ? Olmedo, G., Guo, H H.,* Gregory, B., Nourizadeh, S., Aguilar-Henonin, L., Li, H., An, F., Guzman, P., and Ecker, J.R. , ETHYLENE-INSENSITIVE5 encodes a 5' → 3' exoribonuclease required for posttranscriptional regulation of the EIN3-targeting F-box proteins EBF1/2 , Proc. Natl. Acad. Sci. USA , 2006 , 103: 13286-13293 , (* co-first author) ? Guo, H. and Ecker, J.R. , The ethylene signaling pathway: new insights , Curr Opin Plant Biol , 2004 , 7: 40-49 ? Guo, H and Ecker, J.R , Plant responses to ethylene gas are mediated by H. SCFEBF1/EBF2-dependent proteolysis of EIN3 transcription factor , Cell , 2003 , 115: 667-677 ? Shalitin, D., Yang, H., Mockler T., Maymon, M., Guo, H Whitelam, G., and Lin, C , H., Regulation of Arabidopsis cryptochrome 2 by blue light-dependent phosphorylation , Nature , 2002 , 417: 763-767 ? Guo, H Mockler, T., Duong, H., and Lin, C , SUB1, an Arabidopsis calcium-binding H., protein involved in cryptochrome and phytochrome co-action , Science , 2001 , 291: 487-490.

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            H., Duong, H., Ma, N., and Lin, C. , The Arabidopsis blue light receptor Guo, H cryptochrome 2 is a nuclear protein regulated by a blue light-dependent post-transcriptional mechanism , Plant J. , 1999 , 19: 279-87 ? Mockler, T., Guo, H H., Yang, H., Duong, H., and Lin, C. , Antagonistic actions of the Arabidopsis cryptochromes and phytochrome B in the regulation of floral induction , Development , 1999 , 126: 2073-2082 ? Guo, H H., Yang, H., Mockler, T., and Lin, C. , Regulation of flowering time by Arabidopsis photoreceptors , Science , 1998 , 279: 1360-1363. ? Lin, C., Yang, H., Guo, H Mockler, T., Chen, T., and Cashmore, A.R. , Enhancement H., of blue-light sensitivity of Arabidopsis seedling by a blue light receptor cryptochrome 2 , Proc. Natl. Acad. Sci. USA , 1998 , 95: 2686-2690 ===========================================================

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            纪建国, 教授, 纪建国 教授,博士生导师

            Email: jijg@pku.edu.cn 科研领域描述 本实验室主要兴趣在于: 1. 衰老及退行性病变等细胞增殖异常模型中相关蛋白质群及其调控网络研究 2. 神经细胞核蛋白质的动态修饰与功能研究 3. 体内外蛋白质动态修饰与功能研究 代表性论文 ? Wang Q, He J, Meng L, Liu Y, Pu H, Ji J , A Proteomics Analysis of Rat Liver Membrane Skeletons: The Investigation of Actin- and Cytokeratin-Based Protein Components , J Proteome Res , 2009 , May 8. [Epub ahead of print] ? Liu Y, He J, Wang Q, Ji S, Pu H, Jiang T, Meng L, Yang X, Ji J , Comparative studies of early liver dysfunction in senescence-accelerated mouse using mitochondrial proteomics approaches , Mol Cell Proteomics , 2008 , 7: 1755-1762. ? He J, Liu Y, He S, Wang Q, Pu H and Ji J , Proteomic analysis of a membrane skeleton J, fraction from human liver. , Journal of Proteome Research , 2007 , 6: 3509-3518. ? He J, Liu Y, He S, Wang Q, Pu H, Tong Y and Ji J , Comparison of two-dimensional gel electrophoresis based and shotgun strategies in the study of plasma membrane proteome. , Proteomics-Clinical Application , 2007 , 1: 231-241. ? Diao W, Chen W, Wu Y, Liu P, Xie X, Li S, Shen P, Ji J , Serum, liver, and kidney proteomic analysis for the alloxan-induced type I diabetic mice after insulin gene transfer of naked plasmid through electroporation. , Protoemics , 2006 , 6: 5837-5845. ? He S, Wang Q, He J, Pu H, Yang W and Ji J , Proteomic analysis and comparison of the biopsy and autopsy specimen of human brain temporal lobe. , Proteomics , 2006 , 18: 4987-4996. ? Yang W, Liu P, Liu Y, Wang Q, Tong Y and Ji J , A proteomics profiling of rat pheochromocytoma PC12 cells , Proteomics , 2006 , 10:2982-2990.

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            Chen T, Wang Q, Cui J, Yang W, Shi Q, Ji J and Shen P 2005. . , Induction of apoptosis P, in mice liver by microcystin-LR: a combined transcriptomic, proteomic and simulation strategy , Molecular & Cellular Proteomics , 2005 , 4: 958-974. ? Zhou B, Yang W, Ji J and Ru B , Differential display proteome analysis of PC-12 cells transiently transfected with metallothionein-3 gene , Journal of Proteome Research , 2004 , 3: 126-131. ? Zhao R, Ji J, Tong Y, Pu H, Ru B , Use of serological proteomic methods to find biomarkers associated with breast cancer , Proteomics , 2003 , 3: 433-439. ==================================================

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            蒋争凡, 研究员, 蒋争凡 研究员,博士生导师

            TEL: TEL: 86 - 10 - 6275-7923 Fax: Fax:86 - 10 - 6275-7923 科研领域描述 固有免疫是机体应对于病原微生物感染而进行反击的第一道屏障。 固有免疫的不当调控可引起 许多疾病,包括各种感染性疾病、自身免疫疾病和多种过敏反应等。固有免疫的研究是当前生物学、 医学研究的热点,其中还有很多问题有待于更深入的研究,如细菌、病毒 DNA 在胞浆中的受体是什 么?Mda5/RIG-I/VISA 通路抗病毒的作用机制是什么?不同 I 型干扰素受不同转录因子的调控机制、 固有免疫细胞激活后如何调控不同细胞因子的表达等等还很不清楚。 经典的研究方法:从基因到基因敲除到功能研究已显示出自己的局限性:特定功能或通路中已 知的基因越来越多地被敲除,越来越少的基因可供研究。如何找到新的有重要功能的基因就显得尤 如何找到新的有重要功能的基因就显得尤 为重要。我们将结合功能基因组学的方法,从功能(激活特异基因表达或与固有免疫关键分子相结 为重要 合)入手,寻找固有免疫中调控不同细胞信号通路的新基因,包括已知的,但未曾报道在固有免疫 中有作用的基因,我们将 1)利用 cDNA 文库进行与固有免疫相关的大规模、高通量的基因克隆与功 能筛选;2)利用酵母双杂交寻找与固有免疫关键蛋白相结合的蛋白;3)分离纯化抗病毒通路关键 蛋白并结合肽谱分析寻找与固有免疫关键蛋白相结合的蛋白。我们将利用免疫学,细胞生物学,分 子生物学,生物化学,结构生物学等多种技术来研究这些基因的功能及作用机制。对这些重要免疫 因子表达调控的研究,将为人们了解固有免疫中不同信号分子、不同信号通路的激活机制、不同细 胞因子应对于相同或不同感染而受到的调控机制具有重要意义。同时,通过大规模的筛选,找到特 异性调控某个(类)细胞因子表达的关键分子,可为人类设计新一代的抗炎症、抗病毒药物找到靶 标分子。 主要的研究方案: 1)固有免疫中重要细胞因子的启动子的克隆分析 2)不同感染处理的巨噬细胞 cDNA 表达文库的制备 3)用于文库筛选的细胞系的建立及大规模、高通量筛选 4)筛选获得基因的敲除(Knock-out in somatic cells)及其功能研究 发表文章 ? 1. Sun WX, Li Y, Chen L, Chen HH, You FP, Zhou X, Zhou Y, Zhai ZH, Chen DY, Jiang ZF , ERIS, an ER IFN stimulator, activates innate immune signaling through dimerization. , PNAS , 2009 , In Press ? Xiao H, Qian W, Staschke K, Qian Y, Cui G, Deng L, Ehsani M, Wang X, Qian YW, Chen ZJ, Gilmour R, *Jiang Z Jiang Z, *Li X , Pellino 3b negatively regulates
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            interleukin-1-induced TAK1-dependent NF kappaB activation. , J Biol Chem , 2008 , 283(21):14654-64. , Co-corresponding authors ? Otsuka M, Jing Q, Georgel P, Chen J, Mols J, Kang YJ, Jiang Z Das CS, Pattnaik AK, Z, J. Carlos de la Torre, Beutler B and Han J. , Hypersusceptibility to vesicular stomatitis virus infection in Dicer-deficient mice is due to impaired miR24 and miR93 expression. , Immunity , 2007 , 27: 123-134. ? Beutler B, Jiang Z Georgel P, Crozat K, Croker B, Rutschmann S, Du X and Hoebe K , Z, Genetic Analysis of Host Resistance: Toll-like Receptor Signaling and Immunity at Large. , Annual Review of Immunology , 2006 , 24: 353-89. ? Jiang Z Georgel P, Li C, Choe J, Crozat K, Rutschmann S, Du X, Bigby T, Mudd S, Z, Sovath S,Wilson I A, Olson A, Beutler B , Details of Toll-like receptor:adapter interaction revealed by germ-line mutagenesis. , PNAS , 2006 , 103(29): 10961-66. ? Jiang Z Georgel P, Du X, Shamel L, Sovath S, Mudd S, Huber M, Kalis C, Keck S, Galanos Z, C, Freudenberg M, Beutler B. , CD14 is required for MyD88-independent LPS signaling. , Nat Immunol. , 2005 , 6(6): 565-70. ? Jiang Z Mak TW, Sen G, Li X. , Toll-like receptor 3-mediated activation of NF-kappaB Z, and IRF3 diverges at Toll-IL-1 receptor domain-containing adapter inducing IFN-beta. , PNAS , 2004 , 101(10): 3533-8. ? Jin G, Klika A, Callahan M, Faga B, Danzig J, Jiang Z Li X, Stark GR, Harrington Z, J, Sherf B. , Identification of a human NFkB-activating protein, TAB3. , PNAS , 2004 , 101(7): 2028-33. ? Jiang Z Zamanian-Daryoush M, Nie H, Silva AM, Williams BR, Li X. , Poly (I-C)-induced Z, TLR3-mediated activation of NFkappa B and MAP kinase is through an IRAK-independent pathway employing the signaling components TLR3-TRAF6-TAK1-TAB2-PKR. , J Biol Chem. , 2003 , 278(19): 16713-9. ? Jiang Z Johnson HJ, Nie H, Qin J, Bird TA, Li X. , Pellino 1 is required for Z, interleukin-1 (IL-1)-mediated signaling through its interaction with the IRAK4-IRAK-TRAF6 complex. , J Biol Chem. , 2003 , 278(13): 10952-6. ? Qian Y, Zhao Z, Jiang Z, Li X. , Role of NF kappa B activator Act1 in CD40-mediated signaling in epithelial cells. , PNAS , 2002 , 99(14): 9386-91. ? Jiang Z Ninomiya-Tsuji J, Qian Y, Matsumoto K, Li X. , Interleukin-1 (IL-1) Z, receptor-associated kinase-dependent IL-1-induced signaling complexes phosphorylate TAK1 and TAB2 at the plasma membrane and activate TAK1 in the cytosol. , Mol Cell Biol. , 2002 , 22(20): 7158-67. ? Li X, Commane M, Jiang Z Stark GR. , IL-1-induced NFk B and JNK activation diverge Z, at IRAK. , PNAS , 2001 , 98(8): 4461-5. ==============================================================

            金长文, 教授, 金长文 教授,博士生导师

            北京大学生命科学学院教授, 北京大学化学与分子工程学院教授, 北京核磁共振中心首席科学家 北京大学生命科学学院教授 北京大学化学与分子工程学院教授 北京核磁共振中心首席 Fax: Fax:86 - 10 - 6275-3807 TEL: TEL: 86 - 10 - 6275-6004
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            科研领域描述 本实验室主要兴趣在于: 1. 生物大分子结构与功能关系的研究 2. 生物大分子动力学的核磁共振研究 3. 核磁共振方法学的研究 代表性论文 ? Hongwei Li, Fan Yang, Xue Kang, Bin Xia and Changwen Jin Jin*. , Solution Structures and Backbone Dynamics of Escherichia coli Rhodanese PspE in Its Sulfur-Free and Persulfide-Internediate forms: Implications for the Catalytic Mechanism of Rhodanese , Biochemistry , 2008 , (in press) ? Caifang Yu, You Li, Bin Xia, and Changwen Jin , Solution structure of the cryptic Jin*. mannitol-specific phosphotransferase enzyme IIA CmtB from Escherichia coli. , Biochem. Biophys. Res. Commun. , 2007 , 362(4), 1001-1006, ? You Li, Yunfei Hu, Wenyu Fu, Bin Xia, and Changwen Jin Jin*. , Solution structure of the bacterial chemotaxis adaptor protein CheW from Escherichia coli. , Biochem. Biophys. Res. Commun. , 2007 , 360(4), 863-867 ? Lei Wang, Bin Xia, and Changwen Jin , Solution structure of Escherichia coli HypC , Jin*. Biochem. Biophys. Res. Commun. , 2007 , 361(3), 665-669 ? Fan Yang, Wei Hu, Huimin Xu, Congmin Li, Bin Xia, and Changwen Jin Jin*. , Solution structure and backbone dynamics of an endopeptidase HycI from Escherichia coli: implications for mechanism of the [NiFe] hydrogenase maturation , J. Biol. Chem. , , 2007 , 282(6), 3856-3863 ? Wenyu Fu, Fan Yang, Xue Kang, Xinxin Zhang, You Li, Bin Xia, and Changwen Jin Jin*. , First structure of the polymyxin resistance proteins , Biochem. Biophys. Res. Commun. , 2007 , 361(4), 1033-1037 ? You Li1, Yunfei Hu1, Xinxin Zhang, Huimin Xu, Ewen Lescop, Bin Xia, and Changwen Jin*. Jin , Conformational fluctuations coupled to the thiol-disulfide transfer between thioredoxin and arsenate reductase in Bacillus subtilis , J. Biol. Chem. , 2007 , 282(15), 11078-11083 , ( 1 equal contributions ) ? Xinxin Zhang, Yunfei Hu, Xianrong Guo, Ewen Lescop, You Li, Bin Xia, and Changwen Jin* Jin , The Bacillus subtilis YKUV is a thiol-disulfide oxidoreductase revealed by its redox structures and activity , J. Biol. Chem. , , 2006 , 281(12), 8296-8304 ? Xianrong Guo1, You Li1, Kuan Peng, Yunfei Hu, Congmin Li, Bin Xia, and Changwen Jin , Jin*. Solution structures and backbone dynamics of arsenate reductase from Bacillus subtilis: reversible conformational switch associated with arsenate reduction , J. Biol. Chem., , 2006 , 280(47), 39601-39608 , ( 1 equal contributions ) ? Yunfei Hu, You Li, Xinxin Zhang, Xianrong Guo, Bin Xia, and Changwen Jin , Solution Jin*. structures and backbone dynamics of a flavodoxin MioC from Escherichia coli in both apo- and holo-forms: implications for cofactor binding and electron transfer , J. Biol. Chem. , , 2006 , 281(46), 35454-35466 ? Huimin Xu, Bin Xia, and Changwen Jin , Solution Structure of a Low-Molecular-Weight Jin*. Protein Tyrosine Phosphatase from Bacillus subtilis , J. Bacteriol. , 2006 , 188(4), 1509-1517

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            Ewen Lescop, Yunfei Hu, Huimin Xu, Wei Hu, Juan Chen, Bin Xia, and Changwen Jin , Jin*. The solution structure of Escherichia coli Wzb reveals a novel substrate recognition mechanism of prokaryotic low molecular weight protein tyrosin phophatases , J. Biol. Chem., , 2006 , 281(28), 19570-19577 ? Xue Kang, Yunfei Hu, You Li, Xianrong Guo, Xiaolu Jiang, Luhua Lai, Bin Xia, and Jin*. Changwen Jin , Structural, biochemical and dynamic characterizations of the hRPB8 subunit of human RNA polymerases , J. Biol. Chem., , 2006 , 281(26), 18216-18226 ? Huimin Xu, Peng Zhang, and Changwen Jin*. , Letter to the Editor. NMR assignments Jin of a low molecular weight protein tyrosine phosphatase (PTPase) from Bacillus subtilis , J. Biomol. NMR , 2005 , 31, 363 ? Congmin Li, Xianrong Guo, Zhongchao Jia, Bin Xia, and Changwen Jin Jin*. , Solution Structure of an Antifreeze Protein CfAFP-501 from Choristoneura fumiferana , J. Biomol. NMR , 2005 , 32, 251-256 ? Xinxin Zhang, Caifang Yu, Bin Xia, and Changwen Jin Jin*. , NMR assignment of new thioredoxin-like protein YkuV from Bacillus subtilis , J. Biomol. NMR , 2005 , 32, 258, ? Congmin Li, and Changwen Jin Jin*. , Letter to the Editor: 1H, 13C and 15N resonance assignments of the antifreeze protein cfAFP-501 from spruce budworm at different temperatures , J. Biomol. NMR , 2004 , 30, 101-102 ? Kuan Peng, Xiaodong Su, Bin Xia, and Changwen Jin , Letter to the Editor: 1H, 13C Jin*. and 15N resonance assignments of a Bacillus Subtilis arsenate reductase , J. Biomol. NMR , 2004 , 29,459-460 =================================================

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            孔道春, 教授, 孔道春 教授,博士生导师

            TEL: TEL: 86 - 10 - 6276-0866 Fax: Fax:86 - 10 - 6276-7127 科研领域描述 本实验室主要兴趣在于: 1. 真核细胞 DNA 复制机理。 2. 真核细胞生长分裂及 checkpoint 调控机理。 DNA 复制是细胞生长分裂过程中的一个最基本生化反应。其特点是高度的准确性及严格的调控, 以保证子细胞能从母细胞里得到一套完整的染色体 DNA。 我们的研究专注于真核细胞 DNA 复制起始 的机理及调控。 最近, 我们发现并证明 Sap1 是 DNA 复制起始反应所必需的蛋白。 现正对其功能 及相互作用蛋白进行深入研究。 我们也研究 DNA 复制叉里的生化反应。 Checkpoin 调控细胞生长分裂。 其根本目的是维护细胞内染色体 DNA 完整。 DNA 复制叉的停顿 或 DNA 损伤激活 Checkpoint, 但其机理不清楚。 用生化及遗传的方法,期望能发现一些新的 Checkpoint 激活分子。 我们现正研究 Fen1 及其它一些蛋白在 Checkpoint 中的作用。 代表性论文 ? Daochun Kong Thomas R. Coleman & Melvin L. DePamphilis , Xenopus origin recognition Kong, complex (ORC) initiates DNA replication preferentially at sequences targeted by Schizosaccharomyces pombe ORC. , EMBO J. , 2003 , 22:3441-3450.
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            Daochun Kong and Melvin DePamphilis , Site specific ORC binding, pre-replication complex assembly, and DNA synthesis at S. pombe replication origins. , EMB0 J. , 2002 , 21: 5567-5576 ? Daochun Kong & Melvin L. DePamphilis , Site-specific DNA binding of the Schizosaccharomyces pombe origin recognition complex is determined by the Orc4 subunit , MCB , 2001 , 21: 8095-8103. ? K-Y. Moon, Daochun Kong J-K Lee, S. Raychandhuri, and Jerard Hurwitz. , Kong, Identification and reconstitution of the origin recognition complex from Schizosaccharomyces pombe. , Proc. Natl. Acad. Sci. USA , 1999 , 96: 12367-12372. ? Daochun Kong Jack D. Griffith, and Charles C. Richardson , T7 gene 4 helicase Kong, promotes branch migration through non-homologous, mismatched, or ultraviolet-irradiated DNA regions , Proc. Natl. Acad. Sci. USA , 1997 , 94: 2987-2992 ? Daochun Kong and Charles C. Richardson , Single-stranded DNA binding protein and DNA helicase of bacteriophage T7 mediate homologous DNA strand exchange , EMBO J. , 1996 , 15: 2010-2019. =====================================================

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            教授, 李 毅, 教授,博士生导师

            Fax: Fax:86 - 10 - 6275-4427 TEL: TEL: 86 - 10 - 6275-9690 科研领域描述 本实验室主要兴趣在于: 1. 病毒与宿主的相互作用以及病毒对宿主发育的影响; 2. 宿主对病毒基因的沉默以及病毒对宿主基因沉默的抑制; 3. 抗呼肠孤病毒化合物的筛选及其抗病毒机制研究; 4. 参与宿主抗病反应的细胞因子的结构和功能研究。 代表性论文 ? Feng Zhou, Yingying Pu, Taiyuan Wei, Huijun Liu, Wulan Deng, Chunhong Wei, Biao Ding, Toshihiro Omura and Yi Li , The P2 capsid Protein of the nonenveloped Rice Dwarf Li. Phytoreovirus induces membrane fusion in insect host cells , PNAS , 2007 , 104:19547-19552. ? Huijun Liu, Chunhong Wei, Yongwang Zhong, Yi Li , Rice black-streaked dwarf virus Li. outer capsid protein P10 has self-interactions and forms oligomeric complexes in solution , Virus Research , 2007 , 127:34-42 ? Feng Zhou, Gang Wu, Wulan Deng, Yingying Pu,Chunhong Wei and Yi Li , Interaction of rice dwarf virus outer capsid P8 protein with rice glycolate oxidase mediates relocalization of P8 , FEBS Letter , 2007 , 581:34-40. ? Ming-Rui Duan, Jie Nan, Yu-He Liang, Peng Mao, Lu Lu, Lanfen Li, Chunhong Wei, Luhua Lai, Yi Li and Xiao-Dong Su , DNA Binding Mechanism Revealed by High Resolution Li, Crystal Structure of Arabidopsis thaliana WRKY1 Protein , Nucleic Acid Research , 2007 , 35 (4): 1145-1154

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            Huijun Liu, Chunhong Wei, Yongwang Zhong, Yi Li , Rice black-streaked dwarf virus minor core protein P8 is a nuclear dimeric protein and represses transcription in tobacco protoplasts , FEBS Letter , 2007 , 581:2534-2540 ? Shifeng Zhu, Mao Chen, Yi Li , Interaction of the P2 protein of rice dwarf Li. Phytoreovirus with ent-kaurene oxidase resulting in reducing GA content and this may further cause dwarf in RDV infected rice plants , Plant Physiology , 2005 , 139:1935-1945 ? Leister T., Dahlbeck D., Day, D., Li, Y., Staskwicz, B. , Molecular genetic evidence Y for the role of SGT1 in the intramolecular complementation of Bs2 protein activity in Nicotiana benthamiana , The Plant Cell , 2005 , 17:1268-1278 ? Xuesong Cao, Peng Zhou, Xiaoming Zhang, Shifeng Zhu, Xuehua Zhong, Qi Xiao, Biao Ding, Li Yi , A RNA silencing suppressor from rice phytoreovirus , Journal of Yi. Virology , 2005 , 79:13018-13027 , Highlighted in the issue ? Li Y Bao Y, Wei C, et al, , Rice dwarf phytoreovirus segment S6-encoded nonstructural Y, protein has a cell-to-cell movement function , Journal of Virology , 2004 , 78: 5382-5380. ===================================================

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            教授,博士生导师( Scholar) 林 硕, 教授,博士生导师(Cheung Kong Visiting Scholar)

            Fax: Fax:86 - 10 - 6275-9072 TEL: TEL: 86 - 10 - 6275-9072 E-mail: shuolin@ucla.edu 1977—1981 年 四川农学院,学士 1982—1985 年 中国农业科学院,硕士 1985—1990 年 波士顿大学医学院,博士

            研究方向: 研究方向: 以斑马鱼为主要的模式动物,主要研究领域包括: (1)血细胞和血管细胞的发育, (2)胰脏和器官 的发育, (3)功能基因组学和基因表达的调控, (4)化学生物学。 Publications: Selected Publications: 1. Yang H, Xiang J, Wang N, Zhao Y, Hyman J, Li S, Jiang J, Chen JK, Yang Z, Lin S. 2009. Converse conformational control of smoothened activity by structurally related small molecules. J Biol Chem. 284 (31): 20876-20884. 2. Jiang Z, Song J, Qi F, Xiao A, An X, Liu NA, Zhu Z, Zhang B, Lin S. 2008. Exdpf is a key regulator of exocrine pancreas development controlled by retinoic acid and ptf1a in zebrafish. PLoS Biol. 6 (11): e293. 3. Sumanas S, Gomez G, Zhao Y, Park C, Choi K and Lin S 2008. Interplay among Etsrp/ER71, S, Scl, and Alk8 signaling controls endothelial and myeloid cell formation. Blood 111(9):4500-4510. 4. Song J, Kim HJ, Gong Z, Liu NA and Lin S 2007. Vhnf1 acts downstream of Bmp, Fgf, S, and RA signals to regulate endocrine beta cell development in zebrafish. Dev Biol
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            303(2): 561-575. 5. Wang D, Jao LE, Zheng N, Dolan K, Ivey J, Zonies S, Wu X, Wu K, Yang H, Meng Q, Zhu Z, Zhang B, Lin S and Burgess SM, 2007. Efficient genome-wide mutagenesis of zebrafish genes by retroviral insertions. Proc Natl Acad Sci U S A 104(30): 12428-12433. 6. Yang Z, Jiang H, Zhao F, Shankar DB, Sakamoto KM, Zhang MQ and Lin S 2007. A highly S, conserved regulatory element controls hematopoietic expression of GATA-2 in zebrafish. BMC Dev Biol 7 (1): 97. 7. Kim HJ, Sumanas S, Palencia-Desai S, Dong Y, Chen JN, and Lin S, 2006. Genetic analysis S of early endocrine pancreas formation in zebrafish. Mol Endocrinol 20 (1): 194-203. 8. Sumanas S and Lin S 2006. Ets1-related protein is a key regulator of vasculogenesis S, in zebrafish. PLoS Biol 4 (1): e10. 9. Zhong H, Wu XR, Huang HG, Fan QC, Zhu ZY and Lin S 2006. Vertebrate MAX-1 is required S, for vascular patterning in zebrafish. Proc Natl Acad Sci U S A 103 (45): 16800-16805. 10. Sumanas S, Jorniak T and Lin S 2005. S, Identification of novel vascular endothelial-specific genes by the microarray analysis of the zebrafish cloche mutants. Blood 106 (2): 534-541. ===================================================

            研究员, 刘 东, 研究员,博士生导师

            TEL: TEL: 86 - 10 - 62753189 科研领域描述 1.感觉神经器官的发育 1.感觉神经器官的发育 (Sensory Organ Development) 脊椎动物不同于其它动物的一个重要特征是它们身体前半部有大量丰富的旁侧感觉神经及其依附的 感觉器官。这些器官包括嗅上皮,瞳仁,垂体,三叉神经器官,内耳,鳃弓神经器官,和侧线(鱼 及两栖类特有) 被誉为脊椎动物独有的进化标志。 , 有趣的是, 所有感觉器官的形态前体十分相似: 它们都在外胚层特定的位置加厚形成基板。 经典胚胎学和近代发育生物学研究在过去的一百年间不 断地争论这些感觉器官是否具有发育同源性。至本世纪,争论的焦点为:在脊椎动物发育的原肠胚 期是否存在一个特定的前基板区,并由此分化出头部周围感觉神经系统。我们过去专注于内耳的发 生研究,并于近两年初步揭开从前基板区到内耳命运决定的发育路 径。 我们将深入探讨前基板区 是如何形成和分化的,并比较各种感觉器官形成机制的异同。 2. 听觉形成与再生(Hearing Development and Regeneration) 听觉形成与再生( Regeneration) 据统计,全球逾一亿人口患有各种各样听力丧失的疾病,其中大部分的听力损伤缘于内耳中听力功 能细胞—毛细胞的损伤和无法再生或听觉神经萎缩,死亡。例如,老年性的听力衰退主因为内耳中 毛细胞数量的明显减少:人类初生时每个内耳中具有 1.6 万-3 万个毛细胞,但一经成熟,这些毛细 胞在个体生长发育过程中不再增殖和再生,并因内在病变(遗传及后天获得的疾病,药物中毒等) 和/或环境因素(污染,噪声,随声听等)而不断减少。由于与内耳相连的听觉神经分布和与毛细胞 的配置十分精巧和复杂,所以耳蜗和干细胞移植均产生无法预期的并发症。因此目前治疗听力损伤 最好的方法是希望在病人内耳的正确位置诱导毛细胞的适量再生,及与听觉神经建立准确的联系。 研究毛细胞的发育机制,以及研究毛细胞损伤后再生障碍的机制,对于听觉及其康复的研究至关重 要。我们实验室将毛细胞的发育和再生机理作为研究的长期目标,计划以斑马鱼为主要模式动物, 用遗传,发育及经典胚胎生物学的方法开展研究工作。我们同时还将研究听觉神经的遗传发育,以 探讨其再生可行性和如何与成熟毛细胞建立精准的联接。
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            代表性论文 ? Rotllant J., Liu D. Yan Y., Postlethwait J.H., Westerfield M. and Du S. , Sparc D., (Osteonectin) functions in morphogenesis of pharyngeal skeleton and inner ear , Matrix Biology , 2008 , 27: 561 ? Hans S., Christison J., Liu D. and Westerfield M. , Fgf-dependant otic induction requires competence provided by Foxi1 and Dlx3b , BMC Developmental Biology , 2007 , 7: 5 ? Yan Y-L., Willoughby J., Liu D., Crump J.G., Wilson C., Miller C.T., Singer A., Kimmel D. C., Westerfield M. and Postlethwait J.H. , A pair of Sox: distinct and overlapping functions of zebrafish Sox9 co-orthologs in craniofacial and pectoral fin development , Development , 2005 , 132: 1069 ? Hans S., Liu D. and Westerfield M. , Pax8 and Pax2a function synergistically in otic specification, downstream of the Foxi1 and Dlx3b transcription factors , Development , 2004 , 131: 5091 ? Liu D. Chu H., Maves L., Yan Y-L., Morcos P. A., Postlethwait J.H. and Westerfield D., M. , Fgf3 and Fgf8 dependent and independent transcription factors are required for otic placode specification , Development , 2003 , 130:2213 ? Yan Y-L., Miller C. T., Nissen R., Singer A., Liu D., Kirn A., Draper B., Willoughby D. J., Morcos P. A., Amsterdam A., Chung B., Westerfield M., Haffter P., Hopkins N., Kimmel C. and Postlethwait J. H. , Zebrafish sox9 gene required for cartilage morphogenesis , Development , 2002 , 129: 5065 ? Liu D. Chandy M., Lee S-K. Le Dréan Y., Xiong F., Lee J.W. and Hew C. L. , A zebrafish D., Ftz-F1 (fushi tarazu factor 1) homologue requires multiple sub-domains in the D and E regions for its transcriptional activity , The Journal of Biological Chemistry , 2000 , 275:16758 ? Liu D. Le Dréan Y., Ekker M., Xiong F. and Hew C. L. , Teleost FTZ-F1 homolog and D., its splicing variant determine the expression of salmon gonadotropin II beta gene , Molecular Endocrinology , 1997 , 11: 877 ? Le Dréan Y., Liu D. Wong A. O. L., Xiong F. and Hew C. L. 1996. , Steroidogenic D., factor1 and estradiol receptor act in synergism to regulate the expression of the salmon gonadotropin II beta subunit gene , Molecular Endocrinology , 1996 , 10: 217 ? Liu D. Xiong F. and Hew C. L. , Functional analysis of estrogen-responsive elements D., in Chinook salmon (Oncorhynchus tschawytscha) gonadotropin II beta subunit gene , Endocrinology , 1995 , 136: 3486 ? Xiong F., Liu D. Elsholtz H. P. and Hew C. L. , The Chinook salmon gonadotropin D., II beta subunit gene contains a strong minimal promoter with a proximal negative element , Molecular Endocrinology , 1994 , 8: 771 ? Xie Y., Liu D., Zou J., Li G. and Zhu Z. , Gene transfer via electroporation in fish , D. Aquaculture , 1993 , 111: 207 ==========================================

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            研究员, 刘 磊, 研究员,博士生导师

            Email: leiliu@pku.edu.cn TEL: TEL: 86 - 10-62759691 科研领域描述 My laboratory is interested in two areas of research, aging and cell death. In aging research, we focused on the gene ASIC2 (Acid Sensing Ion Channel 2), which encode an ion channel belongs to the superfamily of DEG/ENaC (Degnerin/Epithelial Sodium Channel). ASIC2 is specifically expressed in mammalian central nervous system. However, its function is largely unknown. To study ASIC2 function in vivo, we generated transgenic flies. We unexpectedly discovered that ASIC2 induced a novel longevity pathway, distinct from the well known pathways such as diet restriction or insulin/IGF pathways. Currently, the mechanism of ASIC2 function is under investigation in fruit flies and nematodes. Cell death during developmental or pathological conditions can be classified into four types based on morphology and genetic pathways: apoptosis, autophagy, programmed necrosis and vesicular necrosis. In general, necrosis occurs at an older age and associated with conditions of massive tissue damages, such as during traumatic brain injury, stroke, seizure and muscular dystrophy. Because of the lack of understanding of vesicular necrosis and paucity of therapies, we generated a genetic model of vesicular necrosis aim at discovering genes and drugs to modify the progression of acute cell injury. Currently, we are characterizing several genes, and searching for more genes using fly genetic screen. Our aim is to build the molecular and cell biological framework of vesicular necrosis. 代表性论文 ? Yishan Sun, Lei Liu, Yehuda Ben-Shahar, Julie S. Jacobs, Daniel F. Eberl, and Michael Ben, J. Welsh , TRPA channels distinguish gravity sensing from hearing in Johnston's organ , PNAS , 2009 , 13606-13611 , [Abstract] , [Full Text] ? David A. Stoltz, Egon A. Ozer, Peter J. Taft, Marilyn Barry, Lei Liu Peter J. Kiss, Liu, et al., , Drosophila are protected from Pseudomonas aeruginosa lethality by transgenic expression of paraoxonase-1. , Journal of Clinical Investigation , 2008 , 118(9): 3123–3131 , [Abstract] , [Full Text] ? Lei Liu Yuhong Li, Runping Wang, Chong Yin, Qian Dong, Huey Hing, Changsoo Kim and Liu, Michael J. Welsh , Drosophila Hygrosensation requires the TRP channels water witch , Nature , 2007 , 450, 294-298 , [Abstract] , [Full Text] ? Lei Liu A. Soren Leonard, David G. Motto, Margaret P. Price, Wayne A. Johnson and Liu, Michael J. Welsh , Contribution of Drosophila DEG/ENaC gene to salt taste , Neuron , 2003 , 39: 133-146 , [Abstract] , [Full Text] ? Lei Liu Olena Yermolaieva, Wayne A. Johnson, Fran?ois M. Abboud, and Michael J. Liu, Welsh , Identification and function of thermosensory neurons in Drosophila larvae , Nature Neuroscience , 2003 , 6(3): 267-273 , [Abstract] , [Full Text] ? Lei Liu Wayne A. Johnson, and Michael J. Welsh , Drosophila DEG/ENaC pocket genes Liu, are expressed in the tracheal system where they may be involved in liquid clearance , PNAS , 2003 , 100(4): 2128-2133 , [Abstract] , [Full Text]

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            Ming-Chen Jiang, Lei Liu Gerry F. Gebhart , Cellular properties of lateral spinal Liu, nucleus neurons in the rat L6-S1 spinal cord , Journal of Neurophysiology , 1999 , 81(6):3078-86 , [Abstract] , [Full Text] ==========================================

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            龙漫远, 教授, 龙漫远 教授,博士生导师

            E-mail: Degrees, Qualifications: Postdoctoral fellow, July 1 1993 - October 31, 1997, joint appointment, Departments of Molecular and Cellular Biology and Organismic and Evolutionary Biology, laboratories of Walter Gilbert and R. C. Lewontin, Harvard University, Cambridge, MA 02138. Ph.D., Genetics, University of California, Davis. Degree received December 12, 1992. Dissertation: The origin and evolutionary mechanisms of new genes. Department of Genetics. Laboratory of Charles Langley. M.S., Genetics, University of California, Davis. 1990. M.S., B.S., Plant Genetics, 1985, Sichuan Agricultural University, Sichuan, China. Plant Quantitative Genetics Institute led by Prof. Zhiren Gao with Profs/ Rong Tingzhao and Ming Daoxu. Postdoctoral research associate, Department of Molecular and Cellular Biology (Walter Gilbert's laboratory) and Department of Organismic and Evolutionary Biology (R.C. Lewontin's laboratory), Harvard University, July 1993 - October 31, 1997. Residential scholar, Harvard University. Advised undergraduate students in various academic issues. 1994 - 1995 academic year. Supervised undergraduate thesis research in Walter Gilbert's lab, April 20, 1994 - August 30, 1996. Research Assistant, Department of Genetics and Center for Population Biology, UC Davis, 1988--1992. Teaching Associate, Principles of Genetics, UC Davis, 1989--1992 Previous Experience Associate Professor with tenure, Department of Ecology and Evolution, Committees on Genetics and Evolutionary Biology, and The College, The University of Chicago. July 1, 2003 - December 31, 2004. Assistant professor, Department of Ecology and Evolution, Committees on Genetics and Evolutionary Biology, and The College, The University of Chicago. November 1, 1997 - June 30, 2003. Postdoctoral research associate, Department of Molecular and Cellular Biology (Walter Gilbert's laboratory) and Department of Organismic and Evolutionary Biology (R.C. Lewontin's laboratory), Harvard University, July 1993 - October 31, 1997. Residential scholar, Harvard University. Advised undergraduate students in various academic issues. 1994 - 1995 academic year. Supervised undergraduate thesis research in Walter Gilbert's lab, April 20, 1994 - August 30, 1996.
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            Research Assistant, Department of Genetics and Center for Population Biology, UC Davis, 1988 - 1992. Teaching Associate, Principles of Genetics, UC Davis, 1989 - 1992 HONORS National Science Foundation (NSF) CAREER award, 2003-2008. David and Lucile Packard Fellowship for Science and Engineering, 1998-2003. Allen G. Marr Prize for the Best Ph.D. Dissertation of University of California, Davis, 1993. Invited to participate in the 11th Frontier of Sciences Symposium of National Science Academy USA for young scientists. Joint / visiting professors or academic advisory councils in other universities/institutes. Publications 1. Zhang J, Dean AM, Brunet F, Long M Evolving protein functional diversity in new M. genes of Drosophila. Proc Natl Acad Sci U S A 101(46): 16246-50 [PubMed] 2. Wang W, Yu H, Long M Duplication-degeneration as a mechanism of gene fission and M. the origin of new genes in Drosophila species. Nat Genet 36(5): 523-7 [PubMed] 3. Hillier LW, Miller W, Birney E, Warren W, Hardison RC, Ponting CP, Bork P, Burt DW, Groenen MA, Delany ME, Dodgson JB, Chinwalla AT, Cliften PF, Clifton SW, Delehaunty KD, Fronick C, Fulton RS, Graves TA, Kremitzki C, Layman D, Magrini V, McPherson JD, Mi. Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution. Nature 432(7018): 695-716 [PubMed] 4. Long M Betran E, Thornton K, Wang W. The origin of new genes: glimpses from the M, young and old. Nat Rev Genet 4(11): 865-75 [PubMed] 5. Betran E, Thornton K, Long M Retroposed new genes out of the X in Drosophila. Genome M. Res 12(12): 1854-9 [PubMed] 6. Wang W, Thornton K, Berry A, Long M Nucleotide variation along the Drosophila M. melanogaster fourth chromosome. Science 295(5552): 134-7 [PubMed] 7. Wang W, Brunet FG, Nevo E, Long M Origin of sphinx, a young chimeric RNA gene in M. Drosophila melanogaster. Proc Natl Acad Sci U S A 99(7): 4448-53 [PubMed] 8. Caceres M, Ranz JM, Barbadilla A, Long M Ruiz A. Generation of a widespread M, Drosophila inversion by a transposable element. Science 285(5426): 415-8 [PubMed] 9. Long M de Souza SJ, Rosenberg C, Gilbert W. Relationship between "proto-splice M, sites" and intron phases: evidence from dicodon analysis. Proc Natl Acad Sci U S A 95(1): 219-23 [PubMed] 10. Long M de Souza SJ, Gilbert W. The yeast splice site revisited: new exon consensus M, from genomic analysis. Cell 91(6): 739-40 [PubMed] 11. Long M, de Souza SJ, Gilbert W. Delta-interacting protein A and the origin of M hepatitis delta antigen. Science 276(5313): 824-5 [PubMed] 12. Long M de Souza SJ, Rosenberg C, Gilbert W. Exon shuffling and the origin of the M, mitochondrial targeting function in plant cytochrome c1 precursor. Proc Natl Acad Sci U S A 93(15): 7727-31 [PubMed]

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            13. de Souza SJ, Long M Schoenbach L, Roy SW, Gilbert W. Intron positions correlate M, with module boundaries in ancient proteins. Proc Natl Acad Sci U S A 93(25): 14632-6 [PubMed] 14. Long M Rosenberg C, Gilbert W. Intron phase correlations and the evolution of the M, intron/exon structure of genes. Proc Natl Acad Sci U S A 92(26): 12495-9 [PubMed] 15. Long M Langley CH. Natural selection and the origin of jingwei, a chimeric processed M, functional gene in Drosophila. Science 260(5104): 91-5 [PubMed] 16. Long M. Evolution of novel genes. Curr Opin Genet Dev 11(6): 673-80 [PubMed] M 17. Long M Thornton K. Gene duplication and evolution. Science 293(5535): 1551 [PubMed] M, de Souza SJ, Long M Klein RJ, Roy S, Lin S, Gilbert W. Toward a resolution of the introns M, early/late debate: only phase zero introns are correlated with the structure of ancient proteins. Proc Natl Acad Sci U S A 95(9): 5094-9 [PubMed] ==========================================

            罗述金, 研究员, 罗述金 研究员,博士生导师

            Email: luo.shujin@gmail.com 科研领域描述 1. 人为活动与野生动物的关系:栖息地片断化对种群遗传多样性的影响,野生动物贸易和消费与新 现传染病的潜在联系等。 2. 地理分布、气候变化等因子在时间和空间尺度上对野生动物种群遗传多样性的影响。 3. 将保护生物学科学方法和理念结合实际应用于中国及亚洲的濒危物种研究与保护。 代表性论文 ? Driscoll CA, Yamaguchi N, Bar-Gal GK, Roca AL, Luo SJ Macdonald DW, and O’Brien SJ, SJ , Mitochondrial phylogeography illuminates the origina of the extinct Caspian tiger and its relationship to the Amur tiger. , PLoS One , 2009 , 4(1): e4125. ? Luo SJ Johnson WE, Martenson J, Antunes A, Martelli P, Uphyrkina O, Traylor-Holzer SJ, K, Smith JLD and O’Brien SJ* , Subspecies genetic assignments of worldwide captive tigers increase conservation value of captive populations. , Current Biology , 2008 , 18(8): 592-596. , [Cover Story] ? Feng L, Lin L, Zhang L, Wang L, Wang B, Luo A, Yang S, Smith JLD, Luo SJ*, and Zhang SJ L , Evidence of wild tigers in southwest China – a preliminary survey of the Xishuangbanna National Nature Reserve. , Cat News , 2008 , 48: 4-6. ? Luo SJ Cai QX, David VA, Zhang L, Martelli P, Lim NTL, Ferrand N, Chin SC, Gaubert SJ, P, Joao Ramos M, O ’ Brien SJ, Antunes A, and Johnson WE , Isolation and characterization of microsatellite markers in pangolins (Mammalia, Pholidota, Manis spp.). , Molecular Ecology Notes , 2007 , 7(2): 269-272. ? Luo SJ*, Johnson WE, David VA, Menotti-Raymond M, Stanyon R, Cai QX, Beck T, Yuhki SJ* N, Pecon-Slattery J, Smith JLD, and O’Brien SJ , Development of Y chromosome intraspecific polymorphic markers in the Felidae. , Journal of Heredity , 2007 , 98(5): 400-413. ? Luo SJ Kim JH, Johnson WE, et al , Phylogeography and genetic ancestry of tigers SJ, (Panthera tigris). , PLoS Biology , 2004 , 2 (12): 2275-2293/e442. , [Cover Story]
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            教授, 吕 植, 教授,博士生导师

            TEL: TEL: 86-10-6275-3434 Fax: Fax:86-10-6276-1035 RESEARCH WORK Lu Zhi is the deputy director of Peking University Center for Nature and Society. The center’s research field includes: to set up a base of scientific expertise in nature conservation and sustainable development, China’s conservation policy research, to implement and promote best conservation practice in China, and to carry out conservation leadership training, and to offer conservation education. By doing this we aim to influence public consciousness and alter consumer behavior. Our research projects includes: the setup of biodiversity database and policy support system; conservation biology study on endangered species (giant pandas, white-headed langurs, Chiese white dolphins, Asiatic black bears, Kiangs, and Chinese desert cats; monitoring mammals using infra-red triggered camera; rapid assessment of biodiversity in hotspot area (RAP), wildlife-human conflicts in Tibet area, and the role of Sacred Lands in biodiversity conservatin – the relationship between biodiversity and culture diversity. These projects distribute in Inner-Mongolia, Qinghai, Tibet, Sichuan, Shaanxi, Yunnan, Guangxi. POSITION HELD ? Full professor, School of Life Sciences, Peking University (2002- present) ? Research Affiliate, School of forestry and Environmental Studies, Yale University (2002-present) ? Visiting faculty at School of Forestry and Environmental Studies, Yale University (2001-2002) ? David Bell Fellow, Center for Population and Development Studies, Harvard University (2000-2001) ? Associate professor, School of Life Sciences, Peking University (1992-2002) ? Visiting Fellow, National Institutes of Health, United of States (1992-1995) EDUCATION ? 1981 –1985, Bachelor’s in biology, Department of Biology, Peking University Biology, ? 1985 – 1988, Master’s in wildlife ecology, Department of Biology, Peking University ? 1988 – 1992, Ph.D. in wildlife ecology, Department of Biology, Peking University COURSES ? Conservation Biology (for undergraduates), Peking University, Autumn semester ? Advances in Ecology Study, (for graduated), Peking University, Autumn semester PUBLICAITONS ? Lü Zhi, 2002, Giant Pandas in the Wild: Saving An Endangered Species. Aperture, New York, NY. pp128 ? Pan Wenshi, Lu Zhi et al . A Chance for Lasting Survival – Ecology, Behavior and Conservation of the Giant Panda. Peking University Press. Beijing (In Chinese) , 2001
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            ? ?

            Lü Zhi and Jenny Springer (Eds.) Tibet’s Biodiversity: Conservation and Management Proceeding of the International Workshop. China Forestry Publishing House, Beijing, 2000 Susan Mainka and Lü Zhi (Eds.) Proceeding of the Feasibility Workshop on the Giant Panda Reintroduction. China Forestry Publishing House, Beijing. 1999 Pan Wenshi, Gao Zhengsheng and Lü Zhi et al. The Giant Panda’s Natural Refuge in Qinling. Peking University Press. p258 (in Chinese) , 1988

            PAPERS DAJUN WANG, SHENG LI, SHAN SUN, HAO WANG, AI CHEN, ? SHENGZHI LI, JUAN LI, AND ZHI LU. 2008. Turning Earthquake Disaster into Long-Term Benefits for the Panda. Conservation Biology 22 (5), 1356–1360 ? Jennifer Turner and Lu Zhi, Building a Green Civil Society in China. In State of the World 2006, World Watch Institute, 2006 ? Schaller, George; Lu Zhi; Wang Hao, Su Tie, Wildlife and nomads in the eastern Chang Tang Reserve, Tibet. Memorie Vol XXXIII pages:59-68; 2005 ? Xu, J., E. T. Ma, D. Tashi, Y. Fu, Z. Lu, and D. Melick. Integrating sacred knowledge for conservation: cultures and landscapes in southwest China. Ecology and Society 10(2): 7. 2005. ? Schaller, George; Lu Zhi; Wang Hao, Su Tie, Wildlife and nomads in the eastern Chang Tang Reserve, Tibet. Memorie Vol XXXIII pages:59-68; 2005 ? Loucks, Colby; Lu Zhi; Eric Dinnerstein; et al., The giant pandas of the Qinling Mountains, China: a case study in designing conservation landscapes for elevational migrants. Conservation Biology,April 2003, ? Lü Zhi, Pan Wenshi, O’Brien, S. et al. Patterns of genetic variation in remaining giant panda populations: an implication to the species conservation. Conservation Biology, Vol.15,No.6, p1596-1607,2001 ? Loucks, Colby; Lu Zhi, et al. Giant Pandas in a Changing Landscape Science Nov.16, 2001 ? Z. Lü, W. Pan, X. Zhu, D. Wang, H. Wang, What has the panda taught us? in Priorities for the Conservation of Mammalian Diversity, A. Entwistle, N. Dunstone, (Eds.) Cambridge University Press, Cambridge, United Kingdom, 2000, pp. 325-334. 2001. ? Lü Zhi The Giant Panda. In D. McDonald et al (Eds). Encyclopedia of World Mammals, Oxford University Press. Oxford, UK,2001. ? Lü Zhi, William Karish and O’Brien, S.J. Genetic diversity in natural populations of Orangutan. Current Biology. 6:354-369,1996 ? O’Brien, S.J., Lü Zhi and Pan Wenshi Panda, people, policy. Nature. 369:169, 1994 =====================================================
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            秦咏梅, 教授, 秦咏梅 教授,博士生导师

            Email: qinym@pku.edu.cn Fax: Fax:86 - 10 - 6275-4427
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            科研领域描述 本实验室主要兴趣在于: 1. 植物超长链脂肪酸合成途径的研究。 2. 超长链脂肪酸调控植物细胞伸长的分子机制研究。 脂肪酸是指一端含有一个羧基的长的脂肪族碳氢链,在生物体内大部分脂肪酸都以脂质形式存 在。超长链脂肪酸是指碳骨架长度超过 20 个碳原子的脂肪酸,在植物体内这类脂肪酸主要集中于细 胞膜鞘脂及植物表面蜡质中,其生物学作用不很清楚。 实验室近几年来以陆地棉和无长绒无短绒突变体为实验材料,通过构建大规模棉花转录组基因 芯片,分离、鉴定和研究棉花纤维伸长相关基因。其中,超长链脂肪酸(VLCFA)合成途径是被鉴定 为一条重要的在纤维发育期显著上调的代谢途径,我们克隆并鉴定编码 3-酮脂酰-CoA 合酶、3-酮脂 酰-CoA 还原酶及烯酰-CoA 还原酶的棉花 cDNA。为验证其生物学功能,将它们互补相对应的酵母突 变体中, 并使用气相色谱质谱联用仪对互补棉花基因的酵母突变体中的脂肪酸进行定性和定量分析。 我们最新的研究发现 VLCFA 通过激活乙烯合成途径不仅能够促进棉纤维伸长发育,而且还能促进拟 南芥茎细胞伸长。这是关于 VLCFA 可能作为植物信号分子的首次报道,对于 VLCFA 如何调控植物发 育的进一步研究具有重要意义。 代表性论文 ? Mei WQ, Qin YM*, Song WQ, Li J, Zhu YX , Cotton GhPOX1 encoding plant class III peroxidase may be responsible for the high level of reactive oxygen species production that is related to cotton fiber elongation , J Genet Genomics , 2009 , 36: 141-150. , (* corresponding author) ? Song WQ, Qin YM*, Saito M, Shirai T, Pujol FM, Kastaniotis AJ, Hiltunen JK, Zhu YX , Characterization of two cotton cDNAs encoding trans-2-enoyl-CoA reductase reveals a putative novel NADPH binding motif , J Exp Bot , 2009 , 60: 1839-1848 ? He XC, Qin YM YM*, Xu Y, Hu CY, Zhu YX , Molecular cloning, expression profiling and yeast complementation of nineteen beta-tubulin cDNAs from developing cotton ovules , J Exp Bot , 2008 , 59: 2687-2695 ? Qin YM , Hu CY, Pang Y, Kastaniotis AJ, Hiltunen JK and Zhu YX , Saturated very-long-chain fatty acids promote cotton fiber and Arabidopsis cell elongation by activating ethylene biosynthesis , Plant Cell , 2007 , 19: 3692-3704 ? Qin YM Pujol FM, Hu CY, Feng JX, Kastaniotis AJ, Hiltunen JK, and Zhu YX , Genetic YM*, and biochemical studies in yeast reveal that the cotton fibre-specific GhCER6 gene functions in fatty acid elongation , J Exp Bot, , 2007 , 58: 473-481 ? Li HB, Qin YM Pang Y, Song WQ, Mei WQ, and Zhu YX , A cotton ascorbate peroxidase YM*, is involved in hydrogen peroxide homeostasis during fibre cell development , New Phytologist , 2007 , 175: 462-471 , ? Qin YM and Zhu YX , A brief summary of major advances in cotton functional genomics and molecular breeding studies in China , Chin Sci Bull , 2007 , 52, 3174-3178 , Review ? Shi, Y.H., Zhu, S.W., Mao, X.Z., Feng, J.X., Qin, Y.M Zhang, L., Cheng, J., Wei. Y.M., L.P., Wang, Z.Y., and Zhu, Y.X , Transcriptome profiling, molecular biological, and physiological studies reveal a major role for ethylene in cotton fiber cell elongation , Plant Cell , 2006 , 18, 651-664 ? Qin YM Pujol FA, Shi YH, Feng JX, Liu YM, Kastaniotis AJ, Hiltunen JK and Zhu YX , YM, Cloning and functional characterization of two cDNAs encoding NADPH-dependent
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            3-ketoacyl-CoA reductases from developing cotton fibers , Cell Research , 2005 , 15: 465-473. ? Qin YM Poutanen MH, and Novikov DK , Substrate specificities of peroxisomal members YM, of short-chain alcohol dehydrogenase superfamily: expression and characterization of dehydrogenase part of Candida tropicalis multifunctional enzyme , J Lipid Res. , 2000 , 41: 93-98 ? Qin YM Haapalainen AM, Kilpelainen SH, Marttila MS, Koski MK, Glumoff T, Novikov YM, DK, and Glumoff T , Human peroxisomal multifunctional enzyme type 2. Site-directed mutagenesis studies show the importance of two protic residues for 2-enoyl-CoA hydratase 2 activity , J Biol Chem, , 2000 , 275: 4965-4972 ? Qin YM Marttila MS, Haapalainen AM, Siivari KM, Glumoff T, and Hiltunen JK , Yeast YM, peroxisomal multifunctional enzyme: (3R)-hydroxyacyl-CoA dehydrogenase domains A and B are required for optimal growth on oleic acid , J Biol Chem, , 1999 , 274: 28619-28625. ? Qin YM Haapalainen AM, Conry D, Cuebas DA, Hiltunen JK, and Novikov DK , Recombinant YM, 2-enoyl-CoA hydratase derived from rat peroxisomal multifunctional enzyme 2: role of the hydratase reaction in bile acid synthesis , Biochem J, , 1997 , 328: 377-382. ? Qin YM, Poutanen MH, Helander HM, Kvist AP, Siivari KM, Schmitz W, Conzelmann E, YM Hellman U, and Hiltunen JK , Peroxisomal multifunctional enzyme of b-oxidation metabolizing D-3-hydroxyacyl-CoA esters in rat liver: molecular cloning, expression and characterization , Biochem J , 1997 , 321: 21-28. =========================================

            瞿礼嘉, 教授, 瞿礼嘉 教授,博士生导师

            Email: qulj@pku.edu.cn TEL: TEL: 86 - 10-6275-3018 Fax: Fax:86 - 10-6275-3339 科研领域描述 学科专长: 学科专长: 植物分子生物学,植物生理生化,植物基因工程 研究方向: 研究方向: 植物激素甲基化修饰的分子生物学研究,拟南芥叶片发育、分枝及雌雄配子体发育的分子生物学研 究,水稻对稻瘟病抗性的分子生物学研究 代表性论文 ? Liu, T., Liu S., Guan H., Ma L., Chen Z., Gu H., Qu L.-J. , Transcriptional profiling L.of Arabidopsis seedlings in response to heavy metal lead (Pb) , Environmental and Experimental Botany , 2009 , 67: 377-386 ? Guo Y., Qin G., Gu H., Qu L.-J. , HCA2, a Dof transcription factor gene, regulates L.interfascicular cambium formation and vascular tissue development in Arabidopsis , The Plant Cell , 2009 , (accepted) ? Liu J., Qu L.-J. , Meiotic and mitotic cell cycle mutants involved in gametophyte L.development in Arabidopsis , Molecular Plant , 2008 , 1(4): 564-574
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            Liu J., Zhang Y., Qin G., Tsuge T., Sakaguchi N., Luo G., Sun K., Shi D., Aki S., Zheng N., Aoyama T., Oka A., Yang W., Umeda M., Xie Q., Gu H., Qu L.-J. , Targeted L.degradation of the cyclin-dependent kinase inhibitor ICK4/KRP6 by RING-type E3 ligases is essential for mitotic cell cycle progression during Arabidopsis gametogenesis , The Plant Cell , 2008 , 20: 1538-1554 ? Li L., Qin G., Tsuge T., Hou X., Ding M., Aoyama T., Oka A., Chen Z., Gu H., Zhao Y., Qu L.-J. , SPOROCYTELESS modulates YUCCA expression to regulate the development L.of lateral organs in Arabidopsis , New Phytologist , 2008 , 179: 751-764 ? Li X., Qin G., Chen Z.L., Gu H., Qu L.-J. , A gain-of-function mutation of L.transcriptional factor PTL results in curly leaves, dwarfism and male sterility by affecting auxin homeostasis , Plant Molecular Biology , 2008 , 66: 315-327. ? Li L., Hou X., Tsuge T., Ding M., Aoyama T., Oka A., Gu H., Zhao Y., Qu L.-J. , The L.possible action mechanisms of indole-3-acetic acid methyl ester in Arabidopsis , Plant Cell Reports , 2008 , 27: 575-584. ? Lin Z., Yin K., Wang X., Liu M., Chen Z., Gu H. and Qu L.-J. , Virus induced gene L.silencing of AtCDC5 results in accelerated cell death in Arabidopsis leaves , Plant Physiol. Biochem. , 2007 , 45: 87-94. ? Yang X.Y., Li J.G., Pei M., Gu H., Chen Z.L., Qu L.-J. , Over-expression of a L.flower-specific transcription factor gene AtMYB24 causes aberrant anther development , Plant Cell Reports , 2007 , 26: 219-228 ? Qin G., Gu H., Ma L., Peng Y., Deng X.-W., Chen Z.L., and Qu L.-J. , Disruption of L.phytoene desaturase gene results in albino and dwarf phenotypes in Arabidopsis by impairing chlorophyll, carotenoid, and gibberellin biosynthesis , Cell Research , 2007 , 17: 471-482 ? Zhang X., Chen Y., Wang Z.-Y., Chen Z.L., Gu H., and Qu L.-J. , Constitutive L.expression of CIR1 (RVE2) affects several circadian-regulated process and seed germination in Arabidopsis , The Plant Journal , 2007 , 51: 512-525 ? Lin Z., Yin K., Zhu D., Chen Z.L., Gu H. and Qu L.-J. , AtCDC5 regulates G2 to M L.transition of cell cycle and is critical for the function of Arabidopsis shoot apical meristems , Cell Research , 2007 , 17: 815-828 ==========================================

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            饶广远, 教授, 饶广远 教授,博士生导师

            Fax: Fax:86 - 10 - 6275-1526 TEL TEL: 86 - 10 - 6275-3035 科研领域描述 本实验室主要兴趣在于: 系统与进化植物学:运用宏观至微观多学科手段,通过对基因到表型、个体到群体多个层次上 遗传变异式样及其成因的综合分析,研究植物类群的起源、分化和散布,探讨它们现代地理分布格 局的成因,揭示植物类群的进化历史及其系统发育关系。 植物居群生物学和保护遗传学:以植物的居群/种群为研究对象,利用群体遗传学的原理和方

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            法,研究目标植物的居群遗传结构、时空动态变化及其成因;探讨物种形成的式样和机制;研究植 物遗传多样性产生、维持和丧失的原因和机制,建立多样性保护和利用的理论基础和技术体系。 代表性论文 ? Liang ZC, Yang J & Rao GY , Development of microsatellite markers in an amphicarpic species, Amphicarpaea edgeworthii Benth. (Leguminosae) , Molecular Ecology Notes , 2007 , 7: 863-865 ? Yang WH, Glover BJ, Rao GY & Yang J , Molecular evidence for multiple polyploidization and lineage recombination in the Chrysanthemum indicum polyploid complex (Asteraceae) , New Phytologist , 2006 , 171: 875-886 ? Zhang Y, Yang J & Rao GY , Comparative aerial and subterranean flower development in Amphicarpaea edgeworthii (Leguminosae: Papilionoideae), an amphicapic legume , International Journal of Plant Sciences , 2006 , 167: 943-949 ? Zhang Y, Yang J & Rao GY , Genetic diversity of an amphicarpic species, Amphicarpaea edgeworthii Benth. (Leguminosae) based on RAPD marker , Biochemical Systematics and Ecology , 2005 , 33: 1246-1257 ? Rao GY Andersson S & Widén B , Pattern of inbreeding depression in a population GY, of Brassica cretica (Brassicaceae): evidence from family-level analyses , Biological Journal of Linnean Society , 2002 , 76: 317-325 ? Rao GY Andersson S & Widén B , Flower and cotyledon asymmetry in Brassica cretica GY, ssp. cretica: Genetic variation and relationship with fitness , Evolution , 2002 , 56: 690-698 ? Rao GY Andersson S & Widén B , Developmental stability in Brassica cretica ssp. GY, cretica: the effect of crossing distance on fluctuating asymmetry in cotyledon morphology , Heredity , 2002 , 88: 197-202 ? Wu, SA, Yang, J & Rao GY , Systematic position of Polygonatum simizui (Convallariaceae) based on morphological, cytological and chloroplast DNA sequence data , Botanical Journal of Linnean Society , 2001 , 137: 291-296 ? He, TH, Rao, GY & You, RL , Reproductive biology of Ophiopogon xylorrhizus (Liliaceae s.l.),an endangered endemic of Yunnan, Southwest China , Australian Journal of Botany , 2000 , 48: 101-107 ? He, TH, Rao, GY GY,You, RL,Ge, S & Zhang, DM. , Genetic diversity of widespread Ophiopogon intermedius: A comparison with endangered O. xylorrhizus , Biological Conservation , 2000 , 96:253-257 ===================================================

            讲席教授, 饶 毅, 讲席教授,博士生导师

            Email: yrao@pku.edu.cn 科研领域描述 本实验室主要兴趣在于: 1. 神经发育的分子机理。 2. 社会行为的分子生物学。

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            极性是细胞的一个基本性质。神经细胞有轴突和树突,它们起不同的作用,树突一般接受信号, 而轴突通常发送信号。如果没有神经细胞的极性,神经系统的信息传递就会紊乱。实验室在分子和 亚细胞水平研究神经细胞极性发生的机理,除了可以帮助基础理解以外,如果能知道怎样形成轴突, 也许可以提示如何在损伤后帮助促进神经纤维再生。目前实验室主要研究调节神经细胞极性的信号 转导通路。 我们通过遗传学途径研究社会行为的分子机理。主要用果蝇和鼠研究同性间争斗、异性间求偶、 和亲子间抚育等行为。用可以定量的实验模型,观察行为的模式和变化。用遗传学方法,控制特定 脑区神经活动,以确立脑中参与特定行为的区域。通过分析基因突变后的行为表型,找到调控行为 的分子。希望理解一个行为执行需要的分子和神经通路,了解行为如何发育,行为发育的分子和细 胞机理。 代表性论文 ? Zhou C, Rao Y, and Rao Yi , A subset of octopaminergic neurons are important for Drosophila aggression , Nature Neurosci , 2008 , 11:1059-1061 , [Full Text] ? Li X, Gao X, Liu G, Xiong W, Wu J, Rao Y , Netrin signal transduction and the guanine nucleotide exchange factor DOCK180 in attractive signaling , Nature Neurosci , 2008 , 11:28-35 , [Full Text] ? Jiang H., Guo W., Liang X.H., and Rao Y. , . Both the establishment and the maintenance of neuronal polarity require active mechanisms: critical roles of GSK-3b and its upstream regulators. , Cell. , 2005 , 120: 123-135. ? Liu G., Beggs H., Jürgensen C., Park H.T., Tang H., Gorski J., Jones K.R., Reichardt L.F., Wu J.Y., and Rao Y. , Netrin requires the focal adhesion kinase and the Src family kinases to induce axon outgrowth and to attract axons , Nature Neurosci , 2004 , 7: 1222. ? Ward M.E., Wu J.Y. and Rao Y , Visualization of spatially and temporally regulated Y. N-WASP activity during cytoskeletal reorganization in living cells. , Proc Natl Acad Sci USA. , 2004 , 101:970-974. ? Zhu Y., Yu T., Zhang X-C, Nagasawa T., Wu J.Y., and Rao Y. , Role of the chemokine SDF-1 as the meningeal attractant for embryonic cerebellar neurons. , Nat Neurosci. , 2002 , 5: 719-720. ? Wu J.Y., Feng L., Park H-T, Havlioglu N., Wen L., Tang H., Bacon K.B., Jiang Z., Zhang X-C, and Rao Y. , Slit, a molecule known to guide axon projection and neuronal migration, inhibits leukocyte chemotaxis induced by chemotactic factors. , Nature. , 2001 , 410: 948-952. ? Wong K., Ren X-R, Huang Y-Z, Xie Y., Liu G., Saito H., Tang H., Wen L., Brady-Kalnay S.M., Mei L., Wu J.Y., Xiong W-C, and Rao Y. , Signal Transduction in Neuronal Migration: Roles of GTPase Activating Proteins and the Small GTPase Cdc42 in the Slit-Robo Pathway. , Cell. , 2001 , 107: 209-221. ? Li H.S., Chen J.H., Wu W., Fagaly T., Yuan W.L., Zhou L., Dupuis S., Jiang Z., Nash W., Gick C., Ornitz D., Wu J.Y., and Rao Y. , Vertebrate Slit, a secreted ligand for the transmembrane protein Roundabout, is a repellent for olfactory bulb axons. , Cell. , 1999 , 96: 807-818. ? Wu W., Wong K., Chen J.H., Jiang Z.H., Dupuis S., Wu J.Y., and Rao Y. , Directional guidance of neuronal migration in the olfactory system by the protein Slit. , Nature. , 1999 , 400: 331-336.
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            Li H.S., Tierney C., Wen L., Wu J.Y. and Rao Y. , A single morphogenetic field gives rise to two retina primordia under the influence of the prechordal mesoderm , Development , 1997 , 124: 603-615 ? Rao Y. Jan L.Y., and Jan Y.N. , Similarity of the product of the Drosophila Y., neurogenic gene big brain to transmembrane channel proteins , Nature , 1990 , 345: 163-167 =================================================

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            苏都莫日根, 教授, 苏都莫日根 教授,博士生导师

            Fax: Fax:86 - 10 - 6275-1526 TEL: TEL: 86 - 10 - 6275-3126 科研领域描述 本实验室主要兴趣在于: 1. 非孟德尔遗传的生物学意义 2. 非孟德尔遗传的分子机理 3. 植物生殖的细胞分子生物学 非孟德尔遗传指线粒体和质体基因组的遗传。人们都知道真核生命的这些细胞质基因组通常呈 现单亲遗传。为什么要单亲遗传?为什么细胞核和细胞质中的遗传物质以不同的方式传递给子代? 非孟德尔遗传对真核生命的形成和进化有怎样的作用和意义?本研究组利用不同的实验模式研究这 些生命的基本问题。 非孟德尔遗传现象发现于近 100 年前,但至今分子调控机制不详。常规的理化诱变方法未能在 模式动植物中获得相关的突变体,说明其分子调控机制的复杂性。本研究组在完成相关细胞学机理 评估的基础上,正在尝试从蛋白质组学的角度探索非孟德尔遗传的基因分子生物学调控机理。 植物的有性生殖是一个复杂的生命活动过程,与非孟德尔遗传有着密切的关联。本研究组同时 对被子植物有性生殖过程中的细胞分化、受精等重要的生命活动过程进行研究。 代表性论文 ? Liu Y, Cui H, Zhang Q Sodmergen , Divergent potentials for cytoplasmic inheritance within the genus Syringa: a new trait associated with speciogenesis , Plant Physiol , 2012 , 136: 2762-2770 ? Hu YF, Zhang Q, Sodmergen , Potential cytoplasmic inheritance in Wisteria sinensis and Robinia pseudoacacia (Leguminosae) , Plant Cell Physiol , 2005 , 46: 1029 -1035 ? Ji X, Zhang Q, Liu Y, Sodmergen , The presence of plastid and absence of mitochondrial DNA in male reproductive cells as evidence for cytoplasmic inheritance in Turnera ulmifolia and Zantedeschia aethiopica , Protoplsma , 2004 , 224: 211-216. ? Liu Y, Zhang Q, Hu YF, Sodmergen , Heterogeneous pollen in Chlorophytum comosum, a species with a unique mode of plastid inheritance intermediate between the maternal and biparental modes , Plant Physiol , 2004 , 135: 193-200 ? Asano T, Yoshioka Y, Kurei S, Sakamoto W, Sodmergen Machida Y , A mutation of the Sodmergen, CRUMPLED LEAF gene that encodes a protein localized in the outer envelope membrane of plastids affects the pattern of cell division, cell differentiation, and plastid division in Arabidopsis , Plant J , 2004 , 38: 448-459.

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            Zhang Q, Liu Y, Sodmergen , Examination of the cytoplasmic DNA in male reproductive cells to determine the potential for cytoplasmic inheritance in 295 angiosperm species , Plant Cell Physiol , 2003 , 44: 941-951 ? Zhang Q, Sodmergen , Cytological evidence for plastid and mitochondrial DNA preservation in mature generative cell in Chlorophytum (Liliaceae) , Protoplasma , 2003 , 221: 211-216 ? Sodmergen Zhang Q, Zhang YT, Sakamoto W, Kuroiwa T , Reduction in amounts of Sodmergen, mitochondrial DNA in the sperm cells as a mechanism for maternal inheritance in Hordeum vulgare , Planta , 2002 , 216: 235-244 =======================================================

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            苏晓东, 教授, 苏晓东 教授,博士生导师

            TEL: TEL: 86 - 10 - 6275-9743 ax: Fax:86 - 10 - 6276-2292 科研领域描述 本实验室主要兴趣在于: 1. 利用 X-射线晶体学手段及其它物理、化学、遗传学技术研究生物学和临床医学的基本问题; 特别是研究物质代谢系统,蛋白质体内合成系统,以及与细胞凋亡和肿瘤的信号传导机制相 关的蛋白质结构。 2. 基于结构的蛋白质工程及药物(蛋白和小分子药物)设计方法。 3. 利用常规及同步辐射 X 光源研究结构生物学方法, 特别是单波长异常散射方法和低分辨率从 头定相位方法; 4. 利用和开发不同宿主系统来表达,纯化那些重要且难于制备的蛋白分子,特别是用于结构和 功能研究的糖蛋白,膜蛋白等。 5. 利用生化及物理学手段研究大分子相互作用及识别机理,特别是功能大分子复合物,细胞黏 附,以及它们在神经系统发育,癌症发生及转移中的作用功能 代表性论文 ? Ming-Rui Duan, Jie Nan, Yu-He Liang, Peng Mao, Lu Lu, Luhua Lai, Yi Li, and Xiao-Dong XiaoSu , DNA Binding Mechanism Revealed by High Resolution Crystal Structure of Arabidopsis thaliana WRKY1 Protein , Nucleic acid research , 2012 , 35(4):1145-54 ? Brostromer E, Nan J. and Su XD , An automated image-collection system for crystallization experiments using SBS standard microplates , Acta Crystallogr D Biol Crystallogr , 2007 , 63:119-25 ? Su XD Liang Y, Li L, Nan J, Brostromer E, Liu P, Dong Y, Xian D , A large-scale, XD, high-efficiency and low-cost platform for structural genomics studies , Acta Crystallogr D Biol Crystallogr , 2006 , 62(8):843-51 ? Yu Y, Liang YH, Brostromer E, Quan JM, Panjikar S, Dong YH, Su XD , A catalytic mechanism revealed by the crystal structures of the imidazolonepropionase from Bacillus subtilis , J. Biol. Chem. , 2006 , 281(48):36929-36 ? Hui Ren, Liya Wang, Matthew Bennett, Yuhe Liang, Xiaofeng Zheng, Fei Lu, Lanfen Li, Jie Nan, Ming Luo, Staffan Eriksson, Chuanmao Zhang, and Xiao-Dong Su , The Crystal Xiao-

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            Structure of human Adenylate Kinase 6: An Adenylate Kinase Localized to the Cell Nucleus , Proc. Natl. Acad. Sci. USA (PNAS) , 2005 , 102:303-308 ? Marcia Alvarez-Fernandez, Yuhe Liang, Magnus Abrahamson and Xiao-Dong Su , Crystal Xiaostructure of human cystatin D, a cysteine peptidase inhibitor with restricted inhibition profile , J. Biol. Chem. , 2005 , 280:18221-18228 ? Selmer, M.; and Su, X.-D , Crystal structure of an mRNA binding fragment of Moorella X.thermoacetica SelB , EMBO J. , 2002 , 21(15):4145-53 ? Bennett, M.; Guan, Z.; Laurberg M. and Su, X-D , Bacillus subtilis arsenate reductase Xis structurally and functionally similar to low molecular-weight protein tyrosine phosphatases , Proc. Natl. Acad. Sci. USA (PNAS) , 2001 , 98:13577-13582 =================================================

            陶乐天, 研究员, 陶乐天 研究员,博士生导师

            Contact E-mail: Tel: +86 10 6275 5206 Address Room 608 New Life Science Building Peking University Beijing, P. R. China, 100871 Previous Experience 2008-Present Center for Bioinformatics, Peking University 2003-2007 Assistant Professor, New Jersey Institute of Technology 2000-2003 Associate Research Scientist, New York University 1997-2000 NSF Postdoctoral fellow, Columbia Unversity 1995-1997 Postdoctoral Research Assistant, Cambridge University Courses Taught Mathematical Modeling and System Biology Research Interests Computational Neuroscience System Biology ==========================================

            王世强, 教授, 王世强 教授,博士生导师

            Email: wsq@pku.edu.cn TEL: TEL: 86 - 10 - 6275-5002 科研领域描述
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            本实验室主要兴趣在于: 1 细胞钙信号转导的微观过程和分子机理; 2 心脏疾病的分子病理机制和生物医学工程; 3 感觉传入诱导的大脑神经网络发育。 钙离子是细胞最古老、作用最广泛的胞内信使。本实验室在激光共聚焦显微成像技术和膜片钳 电生理技术的基础上,建立了单通道光学探测和通道间钙信号转导的研究技术,在国际上居领先优 势。将该技术与分子生物学、功能蛋白组学、电子显微镜技术相配合,可以从分子间相互作用的微 观水平阐述细胞钙信号是如何产生、放大和精细调控的。 分子间钙信号转导是心肌细胞兴奋收缩耦联的核心环节,其异常与心力衰竭、心律失常等重要 疾病密切相关。我们最近在心衰分子病理机制方面取得突破,发现钙信号分子过程的病理改变远早 于细胞和心肌组织的功能变化, 其中关键分子事件是联系细胞膜与肌质网的蛋白 junctophilin 表达 下降。我们正进一步寻找相关的分子调控机制,并在此基础上探索心衰早期诊断新技术和逆转心衰 的生物医学工程方案。 脑和心脏一样是人体最重要的器官,均通过离子通道产生和传递兴奋。本实验室在研究生自由探索 兴趣的驱使下,利用我们强有力的电生理优势和数据处理能力,以“同步发放链”理论框架为基础, 通过分析不同感觉输入条件下神经细胞电活动的时序规律,研究后天经验如何影响大脑皮层神经网 络的发育性构建,已取得重要阶段性发现。 代表性论文 ? Xu M, Zhou P, Xu SM, Liu Y, Feng XH, Bai SH, Bai Y, Hao XM, Han QD, Zhang YY*, Wang SQ* SQ , intermolecular Failure of L-type Ca2+ Channel and Ryanodine Receptor Signaling in Hypertrophy. , PLoS Biol. , 2007 , 5: e21,0203-0211. , 【Highlighted by: Harrison C. Heart disease: Unmasking molecular mechanisms. Nature Reviews Drug Discovery 6: 271, 2007】 ? Tang AH, Chai Z, Wang SQ , Dark rearing alters the short-term synaptic plasticity SQ* in visual cortex , Neurosci. Lett. , 2007 , 422:49-53 ? Zhou C, Tai C, Ye HH, Ren X, Chen JG, Wang, SQ Chai Z* , Interleukin-1b downregulates SQ*, the L-Type Ca channel activity by depressing the expression of channel protein in cortical neurons , J. Cell. Physiol. , 2006 , 206: 799-806 ? Fu Y, Zhang GQ, Hao XM, Wu CH, Chai Z, Wang SQ , Temperature dependence and SQ* thermodynamic properties of Ca sparks in rat cardiomyocytes , Biophys. J. , 2005 , 89: 2533-2541 ? Wang SQ Stern MD, Ríos E, Cheng H , The quantal nature of Ca2+ sparks and in situ SQ, operation of the ryanodine receptor array , Proc. Natl. Acad. Sci. U. S. A. , 2004 , 101: 3979-3984 ? Wang SQ Wei CL, Zhao GL, Brochet DXP, et al , Imaging microdomain Ca2+ in muscle SQ, cells , Circ. Res. , 2004 , 94: 1011-1022 , Review\ ? Liao XD, Tang AH, Chen Q, Jin HJ, Wu CH, Chen LY, Wang SQ , Role of Ca2+ signaling SQ* in initiation of stretch-induced apoptosis in neonatal heart cells , Biochem Biophys Res Commun. , 2003 , 310:405-411 ? Zhu WZ, Wang SQ Khalida C, Yang DM, et al , Linkage of b1-adrenergic stimulation SQ, to apoptotic heart cell death through protein kinase A-independent activation of Ca2+/calmodulin kinase II , J. Clin. Invest. , 2003 , 111, 617-625

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            SQ, Wang SQ Song LS, Xu L, Meissner G, et al , Thermodynamically irreversible gating of ryanodine receptors in situ revealed by stereotyped duration of release in Ca2+ sparks , Biophys. J., , 2002 , 83: 242-251 ? Wang SQ Lakatta EG, Cheng H, Zhou Z , Intracellular calcium homeostasis: adaptive SQ, mechanisms in mammalian hibernators , J. Exp. Biol., , 2002 , 205:2957-2962. , Review ? Cheng H, Wang SQ , Calcium signaling between sarcolemmal calcium channels and ryanodine receptors in heart cells , Frontiers in Bioscience , 2002 , 7:d1867-1878. , Review ? Wang SQ ,Song LS, Lakatta EG, Cheng H , Ca2+ signaling between single L-type Ca2+ channels and ryanodine receptors in heart cells , Nature , 2001 , 410: 592-596. ? Song LS, Wang SQ Lakatta EG, Cheng H , Bata-adrenergic stimulation synchronize SQ, intracellular Ca2+ release during excitation-contraction coupling in cardiac myocytes , Circ. Res. , 2001 , 88: 794-801 ? . Wang SQ Zhou Z , Medical significance of cardiovascular function in hibernating SQ, mammals , Clin. Exp. Pharmacol. Physiol. , 1999 , 26:837-839 , Review =================================================

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            王忆平, 教授, 王忆平 教授,博士生导师

            Email: wangyp@pku.edu.cn TEL: TEL: 86 - 10 - 6275-8490 科研领域描述 本实验室主要兴趣在于: 多年来,本实验室的工作得到国际同行的认可,与欧洲的几个实验室建立并维持着卓有成效 的合作关系,如法国巴斯德研究所、英国帝国理工学院、英国 John Innes 研究所、爱尔兰 Carlow 理工学院、爱尔兰国立大学 Cork 学院、西班牙国家生物技术中心、德国比勒菲尔德大学等。主要得 到国家自然科学基金、国家科技部“863”、“973”项目基金、中法先进合作项目、国家教育部基 金资助。主要工作包括:大肠杆菌及相关细菌中的基因调控网络,尤其是碳代谢和氮代谢的调控偶 联;大肠杆菌及相关细菌中的基因调控机理;植物与微生物相互作用的分子生物学及功能基因组学 研究;生物修复领域的研究(功能基因的分离);在大肠杆菌及相关细菌中大量表达外源基因技术 等。取得的主要成就有,发现原核基因表达调控中碳代谢及氮代谢之间的新的偶联作用及其分子机 理;首次发现“下游激活序列”对固氮基因的调控作用;获得了新型高效抗草苷膦基因,在核酸水 平上与已知基因没有同源性, 在氨基酸水平上也只有 37%左右的同源性, 具有与受专利保护的 EPSPS 基因保守区不同的序列特征。目前世界上 70%的转基因植物含有抗除草剂基因,其中 60%是草甘膦 耐受型 EPSPS 基因,具有很大的应用前景。 代表性论文 ? Tian ZX, Li QS, Buck M, Kolb A and Wang YP , The CRP-cAMP complex and downregulation of the glnAp2 promoter provides a novel regulatory linkage between carbon metabolism and nitrogen assimilation in Escherichia coli , Mol. Microbiol. , 2001 , 41: 911-924. ? Wang YP Kolb A, Buck M, Wen J, O’Gara F and Buc H , CRP interacts with promoter-bound YP, σ54 RNA polymerase and blocks transcriptional activation of the dctA promoter , The EMBO J. , 1998 , 17: 786-796. ==============================================
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            魏丽萍, 教授, 魏丽萍 教授,博士生导师

            北京大学生命科学学院生物信息中心 教授、 主任, 蛋白质工程与植物基因工程国家重点实验室副主 任 Fax:86 - 10 - 6275-4970 Fax: TEL: TEL: 86 - 10 - 6275-5206 工作经历 2004 - now , 副主任 , 蛋白质工程与植物基因工程国家重点实验室 2004 - now , 教授、主任 , 北京大学生命科学学院生物信息中心 2002 - 2004 , 客座助理教授 , 美国斯坦福大学医学院医学系 2001 - 2003 , 共同创始人及首席科学家 , 美国 Nexus Genomics 公司 2000 - 2001 , 研究员 , 美国 Exelixis 公司信息部 教育经历 1994 - 1999 , 理学博士 , 生物医学信息学 , 美国斯坦福大学医学院 1993 - 1994 , 理学硕士 , 应用数学 , 美国布朗大学 1989 - 1993 , 理学学士 , 无线电电子学系 , 中国科学技术大学 代表性论文 ? Li CY, Mao X, Wei L , Genes and (Common) Pathways Underlying Drug Addiction , Plos Computational Biology , 2008 , 4;4(1):e2 ? Zhang W, Zhang Y, Zheng H, Zhang C, Xiong W, Olyarchuk JG, Walker M, Xu W, Zhao M, Zhao S, Zhou Z, Wei L , SynDB: A Synapse protein DataBase based on Synapse Ontology , Nucleic Acids Res. , 2007 , 35:D737-41 ? Ye ZQ, Zhao SQ, Gao G, Liu XQ, Langlois RE, Lu H, Wei L , Finding new structural L. and sequence attributes to predict possible disease association of single amino acid polymorphism (SAP). , Bioinformatics. , 2007 , 23(12):1444-50. ? Kong L, Zhang Y, Ye ZQ, Liu XQ, Zhao SQ, Wei L Gao G , CPC: assess the protein-coding L, potential of transcripts using sequence features and support vector machine , Nucleic Acids Res. , 2007 , 35:W345-9. ? Li CY, Yu Q, Ye ZQ, Sun Y, He Q, Li XM, Zhang W, Luo J, Gu X, Zheng X, Wei L , A L. nonsynonymous SNP in human cytosolic sialidase in a small Asian population results in reduced enzyme activity: potential link with severe adverse reactions to oseltamivir. , Cell Res. , 2007 , 17(4):357-62. , (Cover story) ? Shi YH, Zhu SW, Feng JX, Mao X, Zhang L, Cheng J, Wei LP Wang ZY, Zhu YX , LP, Transcriptome Profiling, Molecular Biological and Physiological Studies Revealed a Major Role of Ethylene in Cotton Fiber Cell Elongation , Plant Cell, , 2006 , 18(3):651-64 ? Zhang Y, Liu XS, Liu QR, Wei L , Genome-wide in silico identification and analysis L. of cis natural antisense transcripts (cis-NATs) in ten species. , Nucleic Acids Res. , 2006 , 34(12):3465-75. ? . Cai Z, Mao X, Li S, Wei L , Genome comparison using Gene Ontology (GO) with statistical testing, , BMC Bioinformatics , 2006 , 7:374.

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            Li J, Li X, Guo L, Lu F, Feng X, He K, Wei L Chen Z, Qu LJ, Gu H. , A subgroup of L, MYB transcription factor genes undergoes highly conserved alternative splicing in Arabidopsis and rice , J Exp Bot. , 2006 , 57(6):1263-73 ? Mao, X., Cai, T., Olyarchuk, J.G., Wei, L , Automated Genome Annotation and Pathway Identification Using the KEGG Orthology (KO) As a Controlled Vocabulary , Bioinformatics , 2005 , 21(19): 3787–93 ===============================================

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            魏文胜, 研究员, 魏文胜 研究员,博士生导师

            TEL: TEL: 86 - 10 - 6275 6762 科研领域描述 感染性疾病一直对人类的健康和生存造成极大的危害。区别于传统的直接针对病原体治疗的策略, 本实验室着重通过宿主来控制和治疗感染性疾病。这种宿主导向的治疗手段特别强调针对已经对传 统治疗产生抗性的病原体,将会为发展治疗感染性疾病提供新的靶点。 研究领域 1) 病原微生物感染的分子机理 2) 发展宿主导向的对抗病原微生物感染的治疗方法 3) 肿瘤细胞转移的分子机制研究 发表文章 ? Lih, C., Wei, W. Cohen, SN. , Txr1: a transcriptional regulator of thrombospondin-1 W., that modulates cellular sensitivity to taxanes. , Genes Dev. , 2006 , 20, 2082-2095. ? Wei, W. Lu, Q., Chaudry, J., Leppla, S., Cohen, SN. , The LDL receptor-related W., protein LRP6 mediates internalization and lethality of anthrax toxin. , Cell , 2006 , 124, 1141-1154. ? Lu, Q., Wei, W. Kowalski, P., Chang, A., Cohen, SN. , EST-based genome-wide gene W., inactivation identifies ARAP3 as a host protein affecting cellular susceptibility to anthrax toxin. , Proc. Natl. Acad. Sci. USA , 2004 , 101, 17246-51. ? Wei, W. Plovanich Jones, A., Deng, W., Jin, Q., Collmer, A., Huang, H., and He, W., SY. , The gene coding for the Hrp pilus structural protein is required for type III secretion of Hrp and Avr proteins in Pseudomonas syringae pv. tomato. , Proc. Natl. Acad. Sci. USA , 2000 , 97, 2247-52. ? Yuan, J., Wei, W. Gopalan, S., Hu, W., Jin, Q., Plovanich-Jones, A., Muncie, L., W., and He, SY. , hrp genes of Pseudomonas syringae. , Molecular Biology of Plant-Microbe Interactions , 2000 , Vol. 5. Eds. Stacey, G. and Keen, N. APS Press, St. Paul, Minnesota, pp1-20. ? He, SY., Wei, W. Yuan, J., Hu, W., Zwiesler-Vollick, J., Thilmony, R., Lee, P., W., Plovanich-Jones, A. , Type III protein secretion and pathogenesis of Pseudomonas syringae pv. tomato DC3000 in Arabidopsis. , In PJGM de Wit, T Bisseling, WJ Stiekema, eds, Biology of Plant-Microbe Interactions , 1999 , Vol. 2. International Soc MPMI, St. Paul, MN, pp 82-87.

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            Roine, E., Wei, W.* , Hrp pilus: an hrp-dependent bacterial surface appendage produced by Pseudomonas syringae pv. tomato DC3000. , Proc. Natl. Acad. Sci. USA , 1997 , 94, 3459-64. , (*Co-first author) ? Gopalan, S., Wei, W. He, SY. , hrp gene-dependent induction of hin1: a plant gene W., activated rapidly by both harpins and the avrPto gene-mediated signal. , Plant J. , 1996 , 10, 591-600. =======================================

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            教授, 夏 斌, 教授,博士生导师

            北京核磁共振中心主任兼首席科学家 Fax: Fax:86 - 10 - 6275-3807 TEL: TEL: 86 - 10 - 6275-8127 科研领域描述 本实验室主要兴趣在于: 利用核磁共振(NMR)技术,结合其它的生化及分子生物学研究技术,研究生物大分子结构、相互作 用,以期揭示其结构与功能关系,揭示其作用的分子机理。目前主要的研究对象包括抑癌基因 HREV 家族的蛋白,SARS 主蛋白酶,植物谷氧还蛋白(Grx)。 代表性论文 ? Nan Zhong, Shengnan Zhang, Peng Zou Jiaxuan Chen, Xue Kang, Zhe Li, Chao Liang, Changwen Jin and Bin Xia , Without its N-Finger, SARS-CoV Main Protease can Form Xia* a Novel Dimer through its C-Terminal Domain , Journal of Virology , 2012 , in press ? Weibin Gong, Gaofeng Cui, Changwen Jin and Bin Xia , 1H, 13C and 15N resonance Xia* assignments of human phosphohistidine phosphatase 1 (PHPT1). , Biomol. NMR Asign , 2007 , 1, 229-231 ? Yingang Feng, Nan Zhong, Nicolas Rouhier, Toshiharu Hase, Masami Kusunoki, Jean-Pierre Jacquot, Changwen Jin & Bin Xia , Structural insight into poplar Xia* glutaredoxin C1 with a bridging iron-sulfur cluster at the active site , Biochemistry., , 2006 , 45, 7998-8008 ? Gaofeng Cui, Beiyan Nan, Jicheng Hu, Yiping Wang, Changwen Jin and Bin Xia , Xia* Identification and solution structures of a Single-domain Biotin/lipoyl Attachment Protein from Bacillus subtilis , J. Biol. Chem., , 2006 , 281, 20598-20607 ? Yingang Feng, Nicolas Rouhier, Jean-Pierre Jacquot and Bin Xia , Letter to the Xia* Editor: 1H, 13C and 15N resonance assignments of reduced glutaredoxin C1 from Populus tremula x tremulides , J. Biomol. NMR , 2005 , 31, 263-264 ==================================

            谢灿, 研究员, 谢灿 研究员,博士生导师

            Email: canxie@pku.edu.cn 科研领域描述
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            INTERESTS RESEARCH INTERESTS Our major interest is to understand how cells communicate with each other and how they sense the environment. Important membrane and cell surface proteins are our major targets. Multiple biochemical and structural biological techniques including negative staining EM, cryo-EM, NMR and X-ray crystallography will be applied. DIRECTIONS AND PROJECTS Our laboratory is pursuing research in three major directions: Plant hormone receptors, Human GPCRs in nervous system, and Virus/host recognition. We are also interested in developing novel eukaryotic expression systems for structural biology studies and pharmaceutical industry. Research Direction 1: Structure and function of Plant Hormone Receptors Project 1: Molecular and Structural biology of plant ethylene receptors Ethylene (C2H4), as a plant growth substance, has the simplest structure. Unlike the rest of the plant hormone compounds, it is a gaseous hormone. Ethylene is produced in all higher plants. It has evolved as the central regulator of cell death programs in plants and is usually associated with flower opening, fruit ripening, stress response, wound healing and host defense. It had been widely believed that all ethylene receptors possess histidine kinase activity and the signal pathway resembles the two-component system, the same way as their sequence homologues in bacteria. We were the first to demonstrate that some of them were actually ser/thr kinases, and suggest that it may serve as an evolutionary bridge connecting the ancient histidine kinases (HKs) in prokaryotes to later evolved ser/thr kinases (STKs) in eukaryotes. We all like to know where we came from. Knowing where we came from can help us go forward. The structure determination of ethylene receptors will address the connections and the differences between HK and STK at atomic level. Other projects will be initiated soon in plant hormone field. proteinResearch direction 2: Structure and function of class C G protein-coupled receptors (GPCR) dimerization Project 2: Structure study, ligand induced dimerization and conformational regulation of human taste receptors type 1 (T1R1, T1R2 and T1R3). Introduction: Taste receptors significantly impact glucose homeostasis, obesity and diabetes. The atomic structure will provide basis for new therapeutic treatments to these diseases and guide new drug design. At the same time, it will also help us on the discovery of new food additives with no or fewer side effects. Research direction 3: Virus/Host Recognition Underdevelopment, coming soon… 代表性论文 ? Xie C Zhu J, Chen X, Mi L, Nishida N, Springer TA , Structure of complement receptor C, type 4, an integrin with an αI domain. (Submitted) , , 2009 , ? Vorup-Jensen, T, Chi, L, Gjelstrup, LC, Jensen, UB, Jewett CA, Xie C Shimaoka M, C, Linhardt RJ, Springer TA , Binding between the integrin αXβ2 (CD11c/CD18) and heparin , J. Biol. Chem. , 2007 , 282:30869-77

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            Nishida N*, Xie C* C**, Shimaoka M, Cheng Y, Walz T, Springer TA , Activation of leukocyte β2 integrins by conversion from bent to extended conformation , Immunity , 2006 , 583-594 (* : Co-first author) ? Chen JF, Takagi J, Xie C Xiao T, Luo BH, Springer TA , The relative influence of C, metal ion binding sites in the I-like domain and the interface with the hybrid domain on rolling and firm adhesion by integrin α4β7 , J. Biol. Chem. , 2004 , 279: 55556-55561 ? Xie C, Shimaoka M, Xiao T, Schwab P, Klickstein LB, Springer TA , The integrin α C subunit leg extends at a Ca2+-dependent epitope in the thigh/genu interface upon activation , Proc. Natl. Acad. Sci. USA , 2004 , 101: 15422-15427 ? Xie C Zhang JS, Zhou HL, Li J, Zhang ZG, Wang DW, Chen SY , Serine/threonine kinase C, activity in the putative histidine kinase-like ethylene receptor NTHK1 from tobacco , Plant J. , 2003 , 33(2):385-393 ? Xie C Zhang ZG, Zhang JS, He XJ, Cao WH, He SJ, Chen SY , Spatial expression and C, characterization of a putative ethylene receptor protein NTHK1 in tobacco , Plant Cell Physiol. , 2002 , 43(7):810-815 ? Zhang JS, Xie C Wu XL, Du BX, Chen SY , Tobacco two-component gene NTHK2 , Chinese C, Science Bulletin , 2001 , 46(7): 574-577 ? Xie C Zhang JS, Chen SY , Protein Phosphorylation and two-component system , C, Progress in Biotechnology , 2001 , 21(6), 9-14 ? Zhang JS *, Xie C* *, Shen YG, Chen SY, 2001, , A two-component gene (NTHK1) encoding a putative ethylene receptor homolog is both developmently- and stress-regulated in tobacco , Thero. Appl. Genet. , 2001 , 102 (6/7): 815-824. (* : Co-first author) ? Wang WM, Zhu LH, Xie R, Xie C Jin L , Morphological and anatomical analysis of a C, floral organ mutant in rice , Acta Botanica Sinica , 2000 , 42(4): 379-382 ? Xie C Zhang JS, Chen SY , Tobacco floral homeotic gene NFBP6 is specifically C, expressed during pollen and ovule development , Science in China(Series C) , 1999 , 42(5),481-484 ? Zhang JS, Xie C Li ZY, Chen SY , Expression of the plasma membrane H+-ATPase gene C, in response to salt stress in a rice salt-tolerance mutant and its original variety, , Theor Appl Genet. , 1999 , 99, (6), 1006-1011 ? Zhang JS, Xie C Liu F, Liu FH, Chen SY, , 1999, , A novel tobacco gene coding for C, a product similar to bacterial two-component regulators , Chinese Science Bulletin , 1999 , 44(11), 1025-1029 =====================================

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            许智宏, 院士,教授, 许智宏 院士,教授,博士生导师

            1942 年 10 月出生于江苏省无锡市。北京大学生命科学学院教授、中国科学院上海植物生理生态研 究所研究员。曾任中国植物生理学会理事长、中国细胞生物学会理事长、中国植物学会副理事长、 中国生物工程学会副理事长。现任国际植物组织培养和生物技术协会主席、UNESCO 人与生物圈中国 委员会主席。 专长:植物发育生物学、植物生物工程。
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            1959 年 9 月至 1965 年 8 月,就读于北京大学生物系植物学专业;毕业后考上上海植物生理研究所 研究生,随后即长期在该所工作;1979 年 8 月至 1981 年 9 月,先后在英国约翰依奈斯研究所和诺 丁汉大学从事研究工作;1983 年 12 月至 1988 年 10 月,任上海植物生理研究所副研究员,副所长; 1988 年 11 月至 1991 年 2 月, 任上海植物生理研究所研究员, 副所长; 1991 年 2 月至 1993 年 10 月, 任上海植物生理研究所所长;1992 年 10 月至 2003 年 2 月任中科院副院长;1999 年 11 月至今任北 京大学校长;1998 年获"有突出贡献的中青年科学家"称号;1990 年获中国科学院自然科学一等奖; 1991 年获国家自然科学三等奖;1994 年获英国 DE MONTFORT 大学荣誉理学博士学位;1994 年获香 港大学荣誉教授;2000 年获英国诺丁大学荣誉理学博士学位;2001 年获香港城市大学荣誉理学博士 学位;2002 年获日本早稻田大学荣誉理学博士学位;2003 年获加拿大 McGill 大学荣誉理学博士学 位。 =====================================

            翟中和, 院士,教授, 翟中和 院士,教授,博士生导师

            电话:+86-10-62767581 Email:zhzhai@pku.edu.cn 工作经历 1930 年生,江苏溧阳人。1956 年毕业于苏联列宁格勒大学生物学系。回国后在北大生物系任 教。后到苏联科学院生物物理研究所进修,并为美国麻省理工学院生物学系访问教授。现任北大生 物系教授、博士生导师,兼任中国细胞生物学会副理事长、中国电子显微镜学会常务理事等职。 翟 中和教授较早建立细胞超微结构技术,首次研制成鸭瘟细胞疫苗,在动物病毒复制与细胞结构 关系 方面取得突出成就。最近,他又在国际上首次证实原始真核细胞存在染色体骨架与核骨架,并在国 内首次建立了非细胞体系核重建的实验模式,首次直观地显示 了重建核的核骨架体系。这些成果受 到国际上的高度重视。 代表性论文 1. Lu ZG, Zhang CM, Zhai Z. 2005, Nucleoplasmin regulates chromatin condensation during apoptosis. PNAS U S A. 102: 2778-2783. 2. Zhao Y, Wu M, Shen Y, and Zhai Z. 2001, Analysis of nuclear apoptotic process in a cell-free system. CMLS 58: 298-306. 3. Zhao Y, Lu Z, Wu M, Han Q, Tao W, and Zhai Z. 2001, Effects of Phosphocreatine on Apoptosis in a Cell-free System. JBC 276(37): 34573-24578. 4. Jiang Z, Hong X, Hu J, and Zhai Z. 2000, Ceramides induce apoptosis in HeLa cells and enhance Cytochrome c-induced apoptosis in Xenopus egg extracts CMLS. 57: 1117-1125. 5. Zhao Y, Liu X, Wu M, Tao W, Zhai Z. 2000, In vitro nuclear reconstitution could be induced in a plant cell-free system. FEBS letters 480: 208-212. 6. Zhao Y, Jiang Z, Sun Y, Zhai Z. 1999, Apoptosis of liver nuclei in the cytosol of carrot cells FEBS Letters 448: 197-200. 7. Sun Y, Zhu H, Zhou J, Dai Y. and Zhai Z. 1999, Menadione-induced apoptosis and the degradation of lamin-like proteins in tobacco protoplasts. CMLS 55: 310-316. 8. Sun Y, Zhao Y, Hong X, Zhai Z. 1999, Cytochrome c release and caspase activation during menadiome-induced apoptosis in plants. FEBS Letters 462: 317-321. 9. Chen Y, Zhang B, Li X, Zhai Z. 1997, Association of DNA with nuclear matrix in in vitro assembled nuclei induced by rDNA from Tetrahymena Shanghaiensis in Xenopus egg extracts.
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            FEBS Letters 413: 449-452. 10. Yang C, Min G, Tong X, Luo Z, Liu Z and Zhai Z. 1995, The assembly of keratins from higher plant cells. Protoplasma 188: 128-132. 11. Yang C, Xin L, and Zhai Z. 1992, Intermediate filaments in higher plant cells and their assembly in a cell free system. Protoplasma 171(1-1): 44-54. 12. Zhai Z, and Penman S. 1987, Alteration in nuclear matrix structural after adenovirus infection. J Virology, 61(4): 1007-1018. =====================================

            教授, 张 博, 教授,博士生导师

            Fax: Fax:86 - 10 - 6275-6185 TEL: TEL: 86 - 10 - 6275-9072 科研领域描述 本实验室主要兴趣在于: 1 以斑马鱼为模式研究脊椎动物早期胚胎发育基因表达调控与器官形成机制,特别是血液与心血管 系统以及胰腺等内脏器官的细胞分化与发育。 2 斑马鱼功能基因组研究。 斑马鱼(Danio rerio)是进行发育生物学研究的理想模式脊椎动物。本实验室以功能基因组研 究手段为切入点,通过筛选斑马鱼中与血液、心血管或胰腺发育相关的突变体与基因,鉴定基因的 表达图谱,并进行基因功能以及表达调控机制的研究。 (1)新基因的筛选:应用以 Tol2 转座子为基础的“增强子诱捕”(enhancer-trap)技术、以 绿色荧光蛋白(GFP)或红色荧光蛋白(RFP)为报告基因大规模筛选具有组织特异性表达图式的转 基因斑马鱼,以及斑马鱼早期胚胎发育相关基因,以建立相应的转基因鱼系与斑马鱼基因表达模式 信息库。迄今已获得 1, 670 种报告基因带有特异表达模式的子代鱼系(F1),其中包括 30 种在胰 腺中特异表达报告基因和 40 多种在血液和/或心血管系统特异表达报告基因的鱼系;鉴定了其中的 118 个 Tol2 插入位点,附近的基因大部分为尚未见报道的新基因。这部分工作由我室与美国加州大 学洛杉矶分校(UCLA)林硕教授的实验室共同合作进行。 (2)突变体的筛选:在与美国国立卫生研究院(NIH)下属的国立人类基因组研究所(NHGRI) Shawn Burgess 教授进行科研合作的基础上,在国内建立了斑马鱼基因插入诱变技术平台;应用反 转录病毒插入诱变技术对斑马鱼进行大规模基因饱和突变筛选,以建立斑马鱼全基因组随机突变文 库与突变体信息库。这部分工作采用了高通量的 LM-PCR 技术,辅以大规模测序与精子冻存保种的全 新策略。目前已筛选到了 244 个基因内的插入,其中大部分为功能未知的新基因。 胰腺是一个既包含内分泌腺又包含外分泌腺的特殊的复合器官, 它的形态发生既涉及到多种信号 通路参与的复杂的细胞分化过程,又存在细胞大量扩增的发育阶段,是研究体内细胞增殖与分化的 良好模式。同时,糖尿病、胰腺癌等严重危害人类健康的疾病与胰腺的发育缺陷与功能失调密切相 关,因而胰腺发育机制的研究具有不言而喻的理论与应用价值。然而,胰腺作为一个内脏器官,由 于其深陷在身体内部而难以进行深入研究,因此胰腺的发育机制研究相对其它器官而言比较滞后。 在上述通过 Tol2 增强子诱捕筛选得到的胰腺被特异标记的鱼系中,GFP 报告基因的可视标记无疑为 我们研究胰腺提供了一个极好的条件。我们将充分发挥上述转基因鱼系与突变体的优势,以胰腺的 发育为模式,研究细胞增殖与分化在胰腺形成中的作用及其机制。 人类对脊椎动物造血系统以及心血管系统的研究已有几百年的历史, 但是它们的发育机制尚未完 全阐明。脊椎动物进化过程中造血过程与心血管的发生是高度保守的,并且造血系统和血管系统共
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            同起源于腹侧中胚层(ventral mesoderm),两者可能具有共同的前体细胞—成血成血管干细胞 (hemangioblast),经进一步诱导分化形成成血细胞(hematopoietic stem cell)及成血管细胞 (angioblast)。丰富的细胞类型与逐级的分化过程使造血系统成为研究体内细胞增殖与分化调控 机制的理想体系。斑马鱼胚胎在受精后 24 小时即可开始心脏跳动与血液循环,并且胚胎通体透明, 血红素呈红色,在体视镜下极易观察;更为宝贵的是,循环系统有缺陷的斑马鱼胚胎依然能够依靠 体表的气体交换存活几天,这样就为我们研究发生缺陷的原因争取了时间。无疑,斑马鱼是开展血 液与心血管发育机制研究的理想材料。 我们将从上述 Tol2 增强子诱捕与反转录病毒插入诱变的筛选 结果中,选择血液和/或心血管系统特异的基因与突变体,深入研究细胞增殖与分化在脊椎动物血液 与心血管系统发育过程中的作用及其调控机制。 代表性论文 ? Wen, L., Wei, W., Gu, W., Huang, P., Ren, X., Zhang, Z., Zhu, Z., Lin, S. and Zhang, B., , Visualization of monoaminergic neurons and neurotoxicity of MPTP in live transgenic zebrafish , Dev. Biol. , 2008 , 314(1): 84-92 ? Wei, W., Wen, L., Huang, P., Zhang, Z., Chen, Y., Xiao, A., Huang, H., Zhu, Z., Zhang, B. and Lin, S , Gfi1.1 regulates hematopoietic lineage differentiation during zebrafish embryogenesis , Cell Res. , 2008 , (in press) ? Wang, D., Jao, L.-E., Zheng, N., Dolan, K., Ivey, J., Zonies, S., Wu, X., Wu, K., Yang, H., Meng, Q., Zhu, Z., Zhang, B., Lin, S. and Burgess, S. M , Efficient B genome-wide mutagenesis of zebrafish genes by retroviral insertions , Proc. Natl. Acad. Sci. USA , 2007 , 104 (30): 12428-12433 ? Zhang J. Z., Gao W., Yang H. B., Zhang B B., Zhu Z. Y. and Xue Y. F , Screening for genes essential for mouse embryonic stem cell self-renewal using a subtractive RNA interference library , Stem Cells , 2006 , 24(12): 2661-2668 ? Liu, B., Lee, K. W., Anzo, M., Zhang, B B., Zi, X., Tao, Y., Shiry, L., Pollak, M., Lin, S. and Cohen, P , Insulin-like growth factor-binding protein-3 inhibition of prostate cancer growth involves suppression of angiogenesis , Oncogene , 2006 , 26(12): 1811-1819 ? Huang, H., Zhang, B., Hartenstein, P. A., Chen, J. N. and Lin, S , NXT2 is required B for embryonic heart development in zebrafish , BMC Dev. Biol., , 2005 , 5(1): 7 ? Sumanas S., Zhang B B., Dai R., Lin S , 15-zinc finger protein Bloody Fingers is required for zebrafish morphogenetic movements during neurulation , Dev Biol., , 2005 , 283(1): 85-96 ? Egli, D., Selvaraj A., Yepiskoposyan H., Zhang B B., Hafen E., Georgiev O. and Schaffner W , Knockout of 'metal-responsive transcription factor' MTF-1 in Drosophila by homologous recombination reveals its central role in heavy metal homeostasis , EMBO J., , 2003 , 22(1): 100-108 ? Zhang B B., Georgiev O., Hagmann M., Günes ?., Faller P., Va?ák M. and Schaffner W , Activation of metal transcription factor-1 (MTF-1) by toxic heavy metals and H2O2 in vitro is modulated by metallothionein , Mol. Cell. Biol., , 2003 , 23(23): 8471-8485 ? Zhang B Egli D., Georgiev O. and Schaffner W , The Drosophila homolog of mammalian B., zinc finger factor MTF-1 activates transcription in response to heavy metals , Mol. Cell. Biol., , 2001 , 21(14): 4505-4514 ===============================================
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            张传茂, 教授, 张传茂 教授,博士生导师

            北京大学生命科学学院副院长, 教育部长江特聘教授 TEL: TEL: 86 - 10 - 6275-7173 科研领域描述 本实验室主要兴趣在于: 1. 细胞核结构与功能 2. 细胞周期调控研究(包括纺锤体装配机理研究,DNA 复制起始调控机理研究,核膜装配机理 研究等) 3. Ran GTPase 在细胞核物质运输和细胞周期调控中的作用 4. 肿瘤细胞生物学(肿瘤细胞增殖调节和增殖控制) 1, 发现 Ran GTPase 控制核膜装配。研究成果以第一作者和负责作者发表在 Science 杂志(2000) 上。获得 Science 编者按积极评价,被国际同行广泛引用,并被国际媒体关注和报道。 (见 Science 杂志 2000 年 5 月 26 日 This Week in Science 栏和 Cell 杂志 2001 年 2 月 9 日(104, 321-324) 评述); 2, (与国际上几个实验室同时)发现 Ran GTPase 控制纺锤体组装。被同行认为是细胞周期研究 领域重要进展之一, 并被广泛引用。 (见 Cell 杂志 2001 年 1 月 12 日 104, 95-106; ( 104, 83-93) 两篇文章引述; 3, 建立了应用相关蛋白质小球和细胞(包括卵细胞)提取物为材料的非细胞体系核膜重建模式。论 文发表在 Current Biology ( 2001, 2002)和 European Journal of Cell Biology (2002)杂志 上。(见 Current Opinion in Cell Biology Volume 15(3) :338-344,June 2003 评述); 4, 发现(2002)核膜物质运输相关蛋白 importin beta 参与核膜装配。(见 2003 年 2 月 21 日 CELL (112 :441-451)和 2003 年 11 月 28 日 SCIENCE (302:1513-1514)引述); 5, 发现 Ran 调控核孔复合体装配(European Journal of Cell Biology 81: 623-633 NOV 2002)。 (见 Nature 杂志 2003 年 8 月 7 日(424: 689-694)引述); 6,发现 RCC1 正确定位在染色体上以产生 Ran-GTP 梯度是人体细胞纺锤体正确装配所需要的 (Moore, Zhang, and PR. Clarke,2002. Current Biology. 12, 1442–1447.)(见 NATURE CELL BIOLOGY 5 (3): 242-248 MAR 2003;5 (6): 505-511 JUN 2003;6 (2): 82-86 FEB 2004 和 Current Opinion in Cell Biology 15(3):338-344 June 2003)引述); 7, 发现 Ran GTPase 通过调控核膜物质运输进而调控 DNA 复制(1998 合作第一作者论文)(见 2003 年 4 月 4 日 Cell 杂志(113, 115-125)引述); 8, 以实验证明(1996)细胞核纤层(Nuclear lamina)对细胞核结构的维持和 DNA 复制起重要调 控作用。(见 1997 年 11 月 Annual Review of Cell and Developmental Biology(13, 669-695) 和 Nature Reviews in Molecular Cell Biology 杂志 2002 年 11 月(3: 848-858)评述); 9, 发现 Nucleoplasin 在非洲爪蟾卵提取物细胞凋亡过程中调控染色质的凝集 (Lu, Z, Zhang C* and Zhai. Z*.2005. Proc. Natl. Acad. Sci. USA (PNAS) 102: 2778–2783). 代表性论文 ? Hui Ren, Liya Wang, Matthew Bennett, Yuhe Liang, Xiaofeng Zheng, Fei Lu, Lanfen Li, Jie Nan, Ming Luo, Staffan Eriksson, Chuanmao Zhang and Xiao-Dong Su , The Crystal Zhang, Structure of human Adenylate Kinase 6: An Adenylate Kinase Localized to the Cell Nucleus. , Proc. Natl. Acad. Sci. USA (PNAS) , 2005 , 102:303-308.

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            Zhigang Lu, Chuanmao Zhang and Zhonghe Zhai* , Nucleoplasmin regulates chromatin Zhang*, condensation during apoptosis. , Proc. Natl. Acad. Sci. USA (PNAS) , 2005 , 102: 2778?C2783 ? Lu, Z, Zhang C and Zhai. Z*. , LDFF, the large molecular weight DNA fragmentation C* factor, is responsible for the large molecular weight DNA degradation during apoptosis in Xenopus egg extracts , Cell Research , 2004 , 14: 134-140 ? Moore, WJ. Zhang, C and PR. Clarke , Targeting of RCC1 to Chromosomes Is Required C. for Proper Mitotic Spindle Assembly in Human Cells. , Current Biology. , 2002 , 12, 1442?C1447. ? Zhang, C and Clarke, P. R. , Role of importin ? in the control of nuclear envelope C., assembly by Ran. , Current Biology. , 2002 , 12: 498?C502. ? . Zhang C., Goldberg, MW, Moore WJ, Allen, TD, Clarke, PR. , Concentration of Ran on chromatin induces decondensation, nuclear envelope formation and nuclear pore complex assembly. , European Journal of Cell Biology , 2002 , 81, 623-633. ? Clarke, P.R., and Zhang, C. , Ran GTPase: a master regulator of nuclear structure and function during the eukaryotic cell division cycle? , Trends Cell Biol. , 2001 , 11, 366?C371. ? Zhang, C*., and Clarke, P. R*. , Chromatin-independent Nuclear Envelope Assembly C induced by Ran GTPase in Xenopus Egg Extracts. , Science. , 2000 , 288:1429-1432. ? Zhang, C C., Hughes, M., Clarke, P. R. , Ran-GTP stablises microtubule asters and inhibits nuclear assembly in Xenopus egg extracts. , J. Cell Sci., , 1999 , 112: 2453-2461 ==========================================

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            郑晓峰, 教授, 郑晓峰 教授,博士生导师

            TEL: TEL: 86 - 62755712 科研领域描述 本实验室主要兴趣在于: 本实验室主要兴趣在于: 1. 人类重要蛋白的结构和生物功能研究。 2. 乙肝病毒 preS 表面抗原及流感病毒神经氨酸酶的功能和结构研究。 3. 蛋白质与核酸相互作用机制的研究。 一些参与基本生物学过程的重要蛋白质的突变所引起的功能失调会导致细胞的非正常发育或功 能丧失,并最终产生包括肿瘤在内的多种疾病,因此,对这些蛋白的结构和功能开展研究具有重要 的生物学意义。研究室主要选取一些与肿瘤等人类重大疾病相关蛋白及一些功能未知的但可能具有 重要生物学意义的新型人类蛋白为靶蛋白,研究其结构和功能的分子基础,研究蛋白质与蛋白质以 及蛋白质与其它生物分子相互识别和相互作用的分子机制、调控网络关系,以及蛋白质功能的失调 与一些重大疾病的发生和发展的关系及分子机理。此外,我们与杨震教授、罗明教授和魏丽萍教授 合作开展流感病毒的抗病毒药物的研究, 旨在确认用于治疗禽流感病毒的感染的有效的小分子药物。 乙型病毒性肝炎是由乙型肝炎病毒(HBV)引起的一种世界性疾病,严重威胁着人类的生命与健 康。据统计,全世界乙型肝炎表面抗原(HBsAg)无症状携带者超过 3 亿,在我国有 1 亿以上。每年都有 大约一百万人死于由慢性肝炎转化为肝硬变或肝癌。 乙型肝炎病毒表面抗原 preS 携带宿主细胞受体

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            识别位点, 在乙肝病毒感染过程中起着重要的作用。 我们希望通过对 preS 的功能和结构的深入研究, 进一步了解 HBV 病毒的入侵和融合作用机制,并且探讨 preS 在诊断、治疗和疫苗方面的应用研究。 此外,研究室还对蛋白质与 RNA 间相互作用机制等开展研究。 代表性论文 ? Zhao YM, Zhang JF, Li HY, Li YY, Ren J, Luo M, Zheng XF , An NADPH sensor protein (HSCARG) downregulates nitric oxide synthesis by association with argininosuccinate synthetase and is essential for epithelial cell viability. , J Biol. Chem. , 2008 , 283(16):11004-11013. , This paper has been selected by Science Signaling as Editor's Choice (Citation: N. R. Gough, NADPH Sensor Regulates NO Production. Sci. Signal. 1, ec142 (2008).) , [Abstract] , [Full Text] ? Meng G, Zhai RT, Liu B, Zheng XF , Identification of a novel nuclear-localized adenylate kinase from Drosophila melanogaster. , Biochemistry (Moscow). , 2008 , 73: 38-43. ? Lian M, Zhou X, Chen B, Li C, Gu X, Luo M, Zheng XF , Identification of the critical regions in HBV preS required for its stability. , J Peptide Science. , 2008 , 14(3): 307-312. , [Abstract] , [Full Text] ? Meng G, Bai X, Green TJ, Luo M, Zheng XF , Crystallization and preliminary X-ray crystallographic studies on SI-CLP, a novel human Glyco_18 domain – containing protein , Protein & Peptide Letter , 2008 , In press ? Chen B, Lian M, Luo M, Zheng XF , A chemical lipid modification of recombinant preS antigen to study the mechanism of HBV attachment to the host cell. , J Biotechnology , 2008 , 137: 8-13 ? Nallagatla SR, Hwang J, Toroney R, Zheng XF Cameron CE, Bevilacqua PC , XF, 5'-triphosphate-dependent activation of PKR by RNAs with short stem-loops. , Science , 2007 , 318: 1455-1458. , [Abstract] , [Full Text] ? Zheng XF Dai XY, Zhao YM, Chen Q, Lu F, Yao DQ, Liu XP, Zhang CM, Gu X, Luo M , XF, Restructuring of the dinucleotide binding fold in a NADP(H) sensor protein. , Proc.Natl.Acad.Sci.USA. , 2007 , 104(21): 8809-8814. , [Abstract] , [Full Text] ? Chen Q, You DL, Liang YH, Su XD, Gu X, Luo M, Zheng XF , Crystal structure of the Thermoanaerobacter tengcongensis hypoxanthine-guanine phosphoribosyltransferase L160I mutant: insights into the inhibitor design. , FEBS J. , 2007 , 274: 4408-4415. , [Abstract] , [Full Text] ? Li CY, Yu Q, Ye ZQ, Sun Y, He QY, Li XM, Zhang WX, Luo JC, Gu X, Zheng XF Wei LP , XF, A Nonsynonymous SNP in Human Cytosolic Sialidase in a Small Asian Population Results in Reduced Enzyme Activity: Potential Link with Severe Adverse Reactions to Oseltamivir. , Cell Research. , 2007 , 17(4):357-362. , [Abstract] , [Full Text] ? Lian M, Zhou X, Wei L, Qiu SH, Zhou T, Li L, Gu X, Luo M, Zheng XF , 3. Serum levels of preS antigen (HBpreSAg) in chronic hepatitis B virus infected patients , Virology J. , 2007 , 4: 93. , [Abstract] , [Full Text] ? Chen Q, Hui R, Sun C, Gu X, Luo M, Zheng XF , Soluble expression, purification, and stabilization of a pro-apoptotic human protein, CARP. , Protein Expression & Purification. , 2006 , 45(2):329-334.

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            Yu YM, Li L, Zheng XF Liang YH, Su XD , Protein Preparation, Crystallization and XF, Preliminary X-ray Analysis of Imidazolonepropionase from Bacillus subtilis. , BBA - Proteins and Proteomics , 2006 , 1764, 153-156. Dai X, Gu X, Luo M, Zheng XF , Protein expression, crystallization and preliminary X-ray crystallographic studies on HSCARG from Homo sapiens. , Protein & Peptide Letter. , 2006 , 13(9):955-957. Zhai RT, Meng G, Zhao YM, Liu B, Zhang GF, Zheng XF , A novel nuclear-localized protein with special adenylate kinase properties from Caenorhabditis elegans. , FEBS Letters. , 2006 , 580: 3811-3817. , [Abstract] Ren H, Wang LY, Bennett M, Liang YH, Zheng XF Lu F, Li LF, Luo M, Eriksson S,. Zhang XF, CM, Su XD , The Crystal Structure of human AK6: An Adenylate Kinase Isoform Localized to the Cell Nucleus. , Proc.Natl.Acad.Sci.USA. , 2005 , 102 (2):303-8. Dai X, Chen Q, Lian M, Zhou Y, Zhou M, Lu SY, Chen YJ, Luo JC, Gu X, Jiang Y, Luo M, Zheng XF , Systematic high-yield production of human secreted proteins in Escherichia coli. , Biochem Biophys Research Commun. , 2005 , 332: 593-601. Li D, Liang YH, Zheng XF Li L, Su XD , Protein preparation, Crystallization and XF, Preliminary X-ray Analysis of YjcG protein from Bacillus subtilis. , Acta Cryst. Sect. F , 2005 , Volume 61(5)Epub Chen Q, Liang YH, Su XD, Gu X, Zheng XF Luo M , Novel IMP Binding in Feedback Zheng XF, Inhibition of Hypoxanthine-Guanine Phosphoribosyltransferase from Thermoanaerobacter tengcongensis. , J. Mol. Biol. , 2005 , 348(5):1199-1210. Ren H, LiangYH, Li R, Ding H, Qiu S, Lu S, An J, Li L, Luo M, Zheng XF Su XD , Protein Zheng XF, preparation, Crystallization and Preliminary X-ray Analysis of human adrenal gland protein AD-004. , Acta Cryst D , 2004 , 60: 1292-1294. Zheng XF and Bevilacqua PC , A novel class of activators of PKR: small double-stranded RNAs with single-stranded tails. , RNA , 2004 , 10:1934-1945. , [Abstract] , [Full Text] Gong W, Liu H, Niu L, Shi Y, Tang Y, Teng M, Wu J, Liang D, Wang D, Wang J, Ding J, Hu H, Huang Q, Zhang Q, Lu S, An J, Liang Y, Zheng X, Gu X, Su XD , Structural genomics efforts at the Chinese Academy of Sciences and Peking University. , J Struct Funct Genomics. , 2003 , 4(23): 137-139. Zheng XF and Bevilacqua PC. , Efficient construction of long DNA duplexes with internal non-Watson-Crick motifs and modifications. , Nucleic. Acids Res. , 2001 , 29(2) e6. Zheng XF and Bevilacqua PC. , Straightening of bulged RNA by the double-stranded RNA-binding domain from the protein kinase PKR. , Proc.Natl.Acad.Sci.USA , 2000 , 97(26):14162-14167. , [Abstract] , [Full Text] Zheng XF, Kobayashi Y, Takeuchi M. , Construction of a low serine-type XF carboxypeptidase producing mutant of Aspergillus oryzae by the expression of antisense RNA and its use as a host for heterologous protein secretion. , Appl. Microbiol. Biotechnology , 1998 , 49:39-44. Zheng XF and Huang BQ , Marker proteins for somatic embryogenesis in several plants. , Acta Bontanica Sinica , 1994 , 36(3):175-180.

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            Zheng XF and Huang BQ , Intranuclear and cytoplasmic inclusions in embryogenic and embryoidal cells of Panax quinquefolius. , Acta Bontanica Sinica , 1994 , 35(6): 422-428. ? Wang L, Zheng XF Zhang G , Rapid propagation of Leymus giganteus L. , Plant XF, Physiology Communications , 1990 , 3:47-48. =============================================

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            朱玉贤, 教授, 朱玉贤 教授,博士生导师

            蛋白质工程及植物基因工程国家重点实验室主任, 博士生导师 Email: zhuyx@water.pku.edu.cn 科研领域描述 基本情况 朱玉贤,北京大学教授,博士生导师,蛋白质工程及植物基因工程国家重点实验室主任。1989 年 12 月在美国康奈尔大学植物科学系获得博士学位,并去华盛顿大学生物系做博士后。91 年 6 月 回国后一直在北京大学生命科学学院工作。97 年 9 月至 98 年 12 月去美国加州伯克利大学做访问教 授。现为中国植物学会副理事长,中国生物工程学会理事,农业生物技术专业委员会副主任; 《植物 学报》《遗传学报》副主编, 、 《科学通报》特邀编辑,植物分子遗传国家重点实验室、农作物基因组 学国家重点实验室学术委员会委员,教育部科学技术委员会生命科学学部委员。1997 年获得国家自 然科学基金委“国家杰出青年科学基金”资助,2001 年被教育部聘为“长江学者” ,2003 年成为国 家自然科学基金委“创新团队”学术带头人。培养博士后 3 名、博士 9 名、硕士 15 名。已在包括世 界权威性科学刊物《植物细胞》《美国科学院院报》《核酸研究》《植物生理学》等杂志上发表 SCI 、 、 、 收录的学术论文 80 余篇。主持多项国家级重大科研项目,并著有全国通用教材《现代分子生物学》 和《分子生物学实验技术》 ,译有《PCR 传奇》和《细胞的起源》等。 主要研究方向 棉纤维细胞伸长机制、植物激素的作用机制、基因表达调控机制 主要研究成果: 主要研究成果: 棉花纤维发育早期特异性表达基因的大规模分离与克隆。 1、棉花纤维发育早期特异性表达基因的大规模分离与克隆。 以陆地棉徐州 142 和它的无长绒无短绒突变体(fuzless-lintless, fl)为实验材料,建立大 规模功能基因筛选与分离克隆的新体系,建立广泛的植物功能基因组学研究、高通量基因组信息分 析和大规模基因功能分析的技术平台, 已建成目前为止世界上规模最大的含有 29,000 多个棉花 cDNA 的基因芯片并利用该芯片分离和克隆了一批在纤维突起、特别是伸长阶段特异性表达的棉花基因, 研 究 并 发 现 发 现 乙 烯 合 成 是 纤 维 伸 长 过 程 中 最 显 著 上 调 的 代 谢 途 径 。筛 选 了 13 个 钙 调 蛋 白 依 赖 型 蛋 白 激 酶 基 因 , 发 现 钙 调 蛋 白 依 赖 型 蛋 白 激 酶 抑 制 剂 TFP 可 以 抑 制 纤 维 细 胞 的 伸 长 ,乙 烯 不 能 恢 复 这 种 抑 制 作 用 ,表 明 CDPK 介 导 的 钙 信 号 通 路 可 能 位 于 外 乙 烯 信 号 下 游 。 现 G hAPX1 基 因 受 H 2 O 2 和 乙 烯 的 调 控 , 加 H 2 O 2 能 够 促 进 纤 维 伸 长 , 发 而 乙 烯 能 促 进 内 源 H 2 O 2 的 释 放 ,表 明 乙 烯 可 能 通 过 CDPK 激 活 N ADP H 氧 化 酶 ,释 放 活 性 氧 ,促 进 纤 维 伸 长 。克 隆 并 鉴 定 了 10 个 VLCFA 延 伸 酶 基 因 ,研 究 了 棉 花 VLCFA 合 成 途 径 关 键 酶 GhKCR 和 GhKCS 的 功 能 , 发 现 VLCFA 能 够 显 著 促 进 棉 纤 维 伸 长 并 诱 导 ACO 基 因 高 表 达 ,促 进 乙 烯 释 放 。外 源 施 加 V LCFA 抑 制 剂 ACE 能 抑 制 纤 维 细 胞 的 伸 长 和 内 源 乙 烯 的 释 放 。AC E 的 抑 制 作 用 可 以 被 乙 烯 所 恢 复 ,但 VLCFA 不 能 恢 复 AVG 的 抑 制作用。 拟南芥转录调控因子家族基因的克隆与研究 2、拟南芥转录调控因子家族基因的克隆与研究
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            克隆了 146 个 AP2/EREBP 转录调控因子家族基因(该家族最多可能有 147 个基因) ,并进行了大 规模基因表达谱分析,找到了一批可能参与调控各种生理过程的受不同环境条件或处理诱导表达的 转录调控因子基因。 通过对拟南芥 ATH1 基因芯片数据的分析, 鉴定了一个受干旱胁迫高水平诱导的 cDNA 片段, 并用 RACE 方法获得了其全长 cDNA。PCR 及定量实时 PCR 分析表明, 该基因在经干旱、 紫外线照射、脱落酸、高盐以及水杨酸等胁迫条件处理后表达量均有显著提高, 特别是干旱处理后 短时间内表达量迅速提高,3 h 后即提高到对照样品的 430 多倍。通过多序列比对和系统进化分析, 将该基因归于 DREB 亚家族。由于该基因编码蛋白含有典型的 AP2/EREBP DNA 结合结构域,并在 N 端 有一段富含谷氨酰氨残基的区域,所以将它命名为 QRAP2 (Glutamine-rich AP2)。凝胶阻滞实验结 果显示, QRAP2 蛋白能够特异结合 DRE 顺式元件序列但不能与 mDRE 和 GCC 等非相关元件结合。酵母 单杂交实验表明,QRAP2 蛋白全长序列或其 N 端 112 个氨基酸与 GAL4 DNA 结合结构域形成的融合蛋 白表现出转录激活功能,而其 C 端 135 个氨基酸与 GAL4 DNA 结合结构域的融合蛋白则没有表现出转 录激活活性。现有资料的分析表明,QRAP2 是 AP2/EREBP 转录因子家族的新成员,可能在干旱胁迫 条件下参与激活相关下游基因的表达。还研究了 TINY2 等多个转录因子的表达特性。正在通过拟南 芥缺失突变体对参与植物抗性反应及植物激素 ABA 信号转导相关基因进行系统性功能研究。 代表性论文 ? Han P, Li Q, Zhu YX , Mutation in the Arabidopsis BARD1 BRCT Domain Releases WUSCHEL Expression from the Organizing Center , The Plant Cell , 2008 , 20:1482-1493 ? Qin YM, Hu CY, Pang Y, Kastaniotis AJ, Hiltunen JK, Zhu YX , Saturated very-long-chain fatty acids promote cotton fiber and Arabidopsis cell elongation by activating ethylene biosynthesis , The Plant Cell , 2007 , 19: 3692-3704 ? Shi YH, Zhu SW, Mao X, Feng JX, Qin YM, Zhang L, Cheng J, Wei L, Wang ZY, Zhu YX , Transcriptome profiling, molecular biological and physiological studies reveal a major role for ethylene in cotton fiber cell elongation , The Plant Cell , 2006 , 18: 651-664 ? Gong, W et al. , Genome-wide ORFeome cloning and analysis of Arabidopsis transcription factor genes , Plant Physiol , 2004 , 135: 773-782 ? Ji SJ, Lu YC, Feng JX, Wei G, Li J, Shi YH, Fu Q, Liu D, Luo JC and Zhu YX , Isolation and Analyses of Genes Preferentially Expressed During Early Cotton Fiber Development by Subtractive PCR and cDNA Array , Nucleic Acids Res. , 2003 , 31: 2534-2543 ? Zhu, Y. X X., Tepperman, J. M., Fairchild, C. and Quail, P. H , Phytochrome B binds with greater apparent affinity than phytochrome A to the basic helix-loop-helix factor PIF3 in a reaction requiring the PAS domain of PIF3 , Proc. Natl. Acad. Sci. USA , 2000 , 97: 13419-13424 ================================================

            朱作言, 院士,教授, 朱作言 院士,教授,博士生导师

            科研领域描述 主要研究成果: 主要研究成果: 鱼类克隆研究:1970 年代在童第周教授指导下,完成了鲤鱼和鲫鱼间的细胞核移植;1990 年代领导 完成了以去核金鱼卵为受体克隆转基因鲤鱼、以去核斑马鱼卵为受体克隆稀有鮈鲫的研究,实 现了低等脊椎动物异种间克隆。 转基因鱼研究:1984 年首创鱼类基因转移研究。构建了“全鱼”基因转移表达载体,相继培育了快
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            速生长转基因鲫鱼、泥鳅和鲤鱼,揭示了外源基因整合的动力学过程,并深入研究了转基因鱼 的生态效应及生物安全问题,为鱼类基因育种奠定了理论基础。 此外,克隆了鱼类 GH 基因, 研究其在脊椎动物早期演化的特殊意义。 发表研究论文 100 多篇,6 次获国家和省部级科技成果奖励。 目前研究方向: 目前研究方向: 1. 主持北京大学遗传与发育生物学研究中心,以斑马鱼、果蝇、线虫等模式动物为材料,探索动 物发育调控的分子机制以及相关的疾病模型的研究:规模性筛选和鉴定模式动物发育相关基因;研 究基因表达图式及其在发育中的功能和可能的致病机制;从分子水平研究细胞分化与发育的调控机 制。. 2. 以水生脊椎动物为材料,通过显微操作技术,探索发育过程中的核质关系以及胞质因子对发育和 形态建成的影响。 代表性论文 ? Dongmei Wang, Li-En Jao, Naizhong Zheng, Kyle Dolan, Jessica Ivey, Seth Zonies?, Xiaolin Wu, Kangmai Wu, Hongbo Yang, Qingchao Meng, Zuoyan Zhu Bo Zhang, Shuo Lin*, Zhu, and Shawn M. Burgess , Efficient genome-wide mutagenesis of zebrafish genes by retroviral insertions , PNAS , 2007 , 104(30):12428-12433 ? 4. Hanbing Zhong, Xinrong Wu, Haigen Huang, Qichang Fan, Zuoyan Zhu and Shuo Lin , Zhu, Vertebrate MAX-1 is required for vascular patterning in zebrafish , Proc Natl Acad Sci U S A , 2006 , 103(45): 16800–16805 ? 5. Jun-zheng Zhang,Wei Gao,Hong-Bo Yang, Bo Zhang, Zuo-yan Zhu and You-fang Xue , ZuoScreening for Genes Essential for Mouse Embryonic Stem Cell Self-Renewal Using a Substractive RNA Interference Library , Stem cell , 2006 , 24;2661-2668 ? Sun YH, Chen SP, Wang YP, Hu W, Zhu ZY , Cytoplasmic impact on cross-genus cloned fish derived from transgenic common carp (Cyprinus carpio) nuclei and goldfish (Carassius auratus) enucleated eggs , Biology of Reproduction , 2005 , 72: 510-515 ? Zhu ZY He L and Chen TT , Primary-structural and evolutionary analyses of the ZY, growth-hormone gene from grass carp (Ctenopharyngodon idellus) , Eur. J. Biochem. , 1992 , 207: 643-648 ? Zhu ZY Li G, He L and Chen S, , Novel gene transfer into the fertilized eggs of ZY, goldfish (Carassius auratus L.1758) , Z.angew.Ichthyol. , 1985 , 1: 31-34 ===============================================

            范六民, 教授, 范六民 教授,博士生导师

            Email: lmfan@pku.edu.cn TEL: TEL: 86 - 10 - 6275-1895 Fax: Fax:86 - 10 - 6275-1895 科研领域描述 本实验室主要兴趣在于: 1 赤霉素信号与逆境及脱落酸信号之间的交互作用。 赤霉素合成或信号转导中某些关键基因的突变导致作物矮化,可增强作物的抗逆能力并提高作 物的产量,故被称为绿色革命基因。对赤霉素信号转导分子机理的解析既具有重要的理论意义又有 广阔的应用前景。我们以拟南芥和水稻作为模式植物,通过基因组学、分子遗传学、生物化学等方
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            法和手段对植物发育过程中赤霉素信号转导过程进行深入和系统的研究,以期发现赤霉素信号转导 过程中的新的组成分子,研究它们与已知蛋白之间交互作用的分子网络。 逆境是影响植物生长发育的主要环境因子。逆境信号转导中脱落酸起非常重要的作用。我们以 拟南芥和水稻作为模式植物进行逆境及脱落酸信号转导途径中关键因子的发掘,研究它们的生物学 功能及其与农艺形状的关系。另外一个研究重点是,用遗传学、生物化学和分子遗传学方法揭示植 物发育及逆境中赤霉素与脱落酸信号之间的交互作用。 代表性论文 ? Wang F, Zhu D, Huang X, Li S, Gong Y, Yao Q, Fu X, Fan LM, Deng XW , Biochemical LM Insights on Degradation of Arabidopsis DELLA Proteins Gained From a Cell-Free Assay System , Plant Cell , 2009 , Aug 28 ? Zhang W, Fan LM (corresponding author). , Actin dynamics regulates voltage-dependent calcium-permeable channels of the Vicia faba guard cell plasma membrane , J Integr Plant Biol , 2009 , 51:912-921. ? Qiao W, Xiao S, Yu L, Fan LM (corresponding author) , . (2009) Expression of a rice gene OsNOA1 re-establishes nitric oxide synthesis and stress-related gene expression for salt tolerance in Arabidopsis nitric oxide-associated 1 mutant Atnoa1 , Environmental and Experimental Botany , 2009 , 65:90-98. ? Qiao W, Fan LM (corresponding author) , Nitric oxide signaling in plant responses to abiotic stresses. , Journal of Integrative Plant Biology , 2008 , 50: 1238– 1246 ? Fan LM Zhang W, Chen JG, Taylor JP, Jones AM, Assmann SM , Abscisic acid regulation LM, of guard-cell K+ and anion channels in Gβ and RGS–deficient Arabidopsis lines , PNAS , 2008 , 105: 8476-8481. ? Zhou J, Wang X, Jiao Y, Qin Y, Liu X, He K, Chen C, Ma L, Wang J, Xiong L, Zhang Q, Fan LM Deng XW , Rice employs largely organ-specific programs in regulation of LM, genome expression in response to drought and high-salinity stresses , Plant Mol Biol , 2007 , 63: 591-608. ? Zhang W, Fan LM, Wu WH , Osmo-sensitive stretch-activated calcium channels in Vicia LM faba guard cells are regulated by actin dynamics , Plant Physiol , 2007 , 143(3):1140-51 ? Fan LM* (corresponding author), Feng X, Wang Y, Deng XW* (corresponding author). , Gibberellin signal transduction in rice , J Integr Plant Biol , 2007 , 49: 731-741. , Review ? Bai SL, Zhong X, Ma L, Zheng W, Fan LM Wei N, Deng XW , A simple and reliable assay LM, for detecting specific nucleotide sequence variations in plants using optical thin-film biosensor chips , Plant Journal , 2007 , 49(2): 354-66 ? Ding L, Fan LM Assmann SM , pgd1, an Arabidopsis thaliana deletion mutant, is LM, defective in pollen germination , Sex Plant Reprod Online , 2007 , 53: 248-263. ? Feng S, Martinez C, Gusmaroli G, Wang Y, Zhou J, Wang F, Chen L, Yu L, Iglesias-Pedraz JM, Kircher S, Schafer E, Fu X, Fan L-M, Deng XW , Coordinated regulation of LArabidopsis development by light and plant hormone gibberellins , Nature , 2007 , 451: 475-479. ? Fan LM Zhao ZX, Assmann SM , Guard cells: a dynamic signaling model , Curr Opin LM, Plant Biol , 2004 , 7: 537-546. , Review
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            Wang YF, Fan LM Zhang WZ, Zhang W, Wu WH , Ca2+-permeable channels in the plasma LM, membrane of Arabidopsis pollen protoplasts are regulated by actin microfilaments , Plant Physiol , 2004 , 136: 3892-3904 ? Coursol S*, Fan LM* (* contributed equally), Le Stunff H, Gilroy S, Speigel S, LM** Assmann SM , Sphingolipid signalling in guard cells involves G proteins , Nature , 2003 , 423:651-654. ? Fan LM Wang YF, Wang H, Wu WH , Outward K+ channels in Brassica chinensis pollen LM, protoplasts are regulated by external and internal pH , Protoplasma , 2003 , 220:143-152. ? Fan LM Wang YF, Wang H, Wu WH , In vitro Arabidopsis pollen germination and LM, characterization of the inward potassium currents in Arabidopsis pollen grain protoplasts , J Exp Bot , 2001 , 52: 1603-1614 ? Fan LM Wu WH , External pH regulates the inward K+ channels in the plasma membranes LM, of Brassica pollen protoplasts , Prog Nat Sci , 2000 , 10: 68-73. ? Fan LM Wu WH, Yang HY , Identification and characterization of the K+ inward currents LM, in the plasma membranes of Brassica pollen protoplasts , Plant Cell Physiol , 1999 , 40: 859-865. =============================================

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            滕俊琳, 副教授, 滕俊琳 副教授,博士生导师

            Email: junlinteng@pku.edu.cn TEL: TEL: 86 - 10 - 62767044 科研领域描述 本实验室主要兴趣在于: 1. 微管相关蛋白与神经细胞的极性构建及人类疾病 2. 分子马达与神经细胞内的物质运输 3. 神经系统发育及细胞分化的分子机制 代表性论文 ? 1. Li H, Guo Y, Teng J, Ding M, Yu CH Albert and Chen J , 14-3-3 (gamma) affects dynamics and integrity of glial filaments by binding to phosphorylated GFAP , J Cell Sci , 2006 , 119: 4452-4461 ? 2. Teng J Tatemitsu R, Yosuke T, Yosuke T, Takao N, Motoyuki H, Ashok B K and Nobutaka J, H , KIF3 motor is essential for organization of proliferative cells in developing brain , Nat Cell Biol , 2005 , 7: 474-482 ? 3. Wong W-C R, Setou M, Teng J Takei Y and Hirokawa N , Overexpression of motor J, protein KIF17 enhances spatial and working memory in transgenic mice , Proc Natl Acad Sci USA , 2002 , 99: 14500-14505 ? 4. Harada A, Teng J Takei Y, Oguchi Kand Hirokawa N , MAP2 is required for PKA J, anchoring in dendrites and proper PKA signal transduction , J Cell Biol , 2002 , 158: 541-549

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            5. Teng J Takei Y, Harada A, Nakata T, Chen J and Hirokawa N , Synergistic effects J, of MAP2 and MAP1B knockout in neuronal migration, dendritic outgrowth, and microtubule organization , J Cell Biol , 2001 , 155: 65-76 ? 6. Takei Y, Teng J Harada A and Hirokawa N , Defects in Axonal Elongation and Neuronal J, Migration in Mice with Disrupted Tau and map1b Genes , J Cell Biol , 2000 , 150: 989-1000 =================================

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            王戎疆, 副教授, 王戎疆 副教授,博士生导师

            TEL: TEL: 86 - 10 - 62758121 科研领域描述 研究兴趣主要集中在种群生态学和分子生态学 栖息地破坏是物种绝灭的最主要的原因之一。日益加剧的人类活动将生物原有栖息地分割成小 的斑块,大量物种就只能生活于这样片段化的栖息地中。小的斑块只能维持有限数量的种群,面临 很高的绝灭风险,而现有种群迁出个体可以在空的栖息地中重新建立种群,物种可以在绝灭和重定 殖的平衡中得以维持,即以集合种群(metapopulation)形式存在。栖息地片段化将给生活于其中 的物种带来怎样的影响,同时物种又是如何适应栖息地片段化并得以续存,这是我们现在所关注的 主要问题,这对探讨物种的保护策略及保护区设计原则具有重要意义。本研究组以庆网蛱蝶 (Melitaea cinxia)为主要研究对象,通过和芬兰庆网蛱蝶种群的比较研究,探讨不同片段化程度 下集合种群结构动态及其影响因素,从而分析栖息地片段化对庆网蛱蝶的影响,以及庆网蛱蝶的适 应机制。目前,主要涉及集合种群动态对迁移能力快速进化的影响、近交衰退对集合种群动态的影 响、种群结构和遗传结构、雄蝶的求偶及交配策略、蝴蝶的高温适应及温度调节等方面。 将分子生物学技术应用到生态学研究中,是对生态学研究的有利补充,这就是分子生态学。利 用分子生物学技术可以研究物种的系统发育、种群的遗传分化、个体的亲缘关系等。研究对象除了 庆网蛱蝶外,还有尾蛱蝶、网蛱蝶等昆虫,以及亚洲黑熊等大型动物(和其他组的合作研究)。 代表性论文 ? Qian WANG, Shuqiang LI, Rongjiang WANG Pierre PAQUIN. , Phylogeographic analysis WANG, of Pimoidae (Arachnida: Araneae) inferred from mitochondrial cytochrome c oxidase subunit I and nuclear 28S rRNA gene regions. , J Zool Syst Evol Res , 2008 , 46(2), 96–104 ? Kun XUE, Su DENG, Rongjiang WANG Fengming YAN, Chongren XU. , Leaf surface factors WANG, of transgenic Bt cotton associated with the feeding behaviors of cotton aphids: A case study on non-target effects. , Sci China Ser C-Life Sci , 2008 , 51(2): 145-156 ? 殷海娣,黄翠虹,薛堃,王戎疆 王戎疆,闫凤鸣 , 昆虫唾液成分在昆虫与植物关系中的作用 , 昆 王戎疆 虫学报 , 2006 , 49(5): 843-849 ? Yu LONG, Hong WAN, Fengming YAN, Chongren XU, Guangchun LEI, Shaowen LI, and WANG NG. Rongjiang WANG , Glacial Effects on Sequence Divergence of Mitochondrial COII of Polyura eudamippus (Lepidoptera: Nymphalidae) in China. , Biochemical Genetics , 2006 , 44(7/8):361-377. ? Cuihong Huang, Xiaoying Wang, Rongjiang Wang Kun Xue, Fengming Yan, Chongren Xu. , Wang, Distribution and variations of three 1,4-benzoxazin-3-ones in maize induced by the

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            Asian corn borer Ostrinia furnacalis (Guenee). , Zeitschrift Fur Naturforschung C , 2006 , 61(3-4):257-262. ? 陈洁君,王义飞,雷光春,王戎疆 王戎疆,徐汝梅. , 栖息地质量对两种网蛱蝶集合种群结构和分 王戎疆 布的影响. , 昆虫学报 , 2004 , 47(1): 59-66. ? 王戎疆 王戎疆,万宏,龙玉,李绍文,雷光春. , 利用线粒体 COII 基因序列对中国尾蛱蝶属系统 分化的研究(鳞翅目:蛱蝶科). , 昆虫学报 , 2004 , 47(2): 243-247 ? Rongjiang Wang Yifei Wang, Jiejun Chen, Guangchun Lei, Rumei Xu. , Contrasting Wang, movement patterns in two species of checkerspot butterflies, Euphydryas aurinia and Melitaea phoebe, in the same patch network. , Ecological Entomology , 2004 , 29, 367-374 ? 王戎疆 万宏; 雷光春; 李绍文 , 我国不同地区二尾蛱蝶线粒体细胞色素氧化酶Ⅱ的 DNA 王戎疆; 序列差异 , 北京大学学报(自然科学版) , 2003 , 39(6):775-779 ? 王义飞, 陈洁君, 王戎疆, 徐汝梅. , 两种共存网蛱蝶的不同集合种群结构及动态. , 王戎疆 雷光春, 科学通报 , 2003 , 48(9): 940-946 ? Rongjiang WANG Yifei WANG, Guangchun LEI, Rumei XU and Jodie PAINTER. , Genetic WANG, differentiation within metapopulations of Euphydryas aurinia and Melitaea phoebe in China. , Biochemical Genetics , 2003 , 41(3/4): 107-118 ? Rongjiang WANG, Yu LONG. , An improved COII primer specific for Lepidoptera. , WANG Biochemical Genetics , 2003 , 41(1/2): 57-60 ? 罗晨,姚远,王戎疆 王戎疆,阎凤鸣,胡敦孝,张芝利. , 利用 mtDNA COI 基因序列鉴定我国烟粉 王戎疆 虱的生物型 , 昆虫学报 , 2002 , 45(6): 759-763 ? Rongjiang WANG Jodie N PAINTER and Ilkka HANSKI. , Amplification of DNA markers WANG, from scat samples of the least weasel Mustela nivalis nivalis. , Acta Theriologica , 2002 , 47(4):425-431 ? 王戎疆 , 粪便 DNA 分析技术在动物生态学中的应用 , 动物学报 , 2001 , 47(6): 699~703 ====================================

            副教授, 张 研, 副教授,博士生导师

            TEL: TEL: 86 - 10 - 62754880 教育经历 ? 1999 - 2003 , 理学博士 , Neurology and Neurosurgery , McGill University ? 1997 - 1999 , 理学硕士 , Neuroscience , Carleton University ? 1993 - 1997 , 理学学士 , Psychology , Peking University 发表书目 ? Zhang Y and LeBlanc A , Microinjection of proteins, peptides and genes in human neuronal apoptosis study. In: Neuromethods, volume 37: Apoptosis Techniques and Protocols, 2nd Edition. , Humana Press, Totowa, New Jersy, USA. , 2002 , 发表文章 ? Zhang Y and Yu L , Single-cell microinjection technology in cell biology , BioEssays , 2008 , 30: 606-610. ? Li M, Chen L, Lee D, Yu L and Zhang Y , The Role of Intracellular Amyloid β in Alzheimer’s Disease , Prog Neurobiol , 2007 , 17: 233-236.
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            ? ?

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            Y, Zhang Y Beitel L, Goodyer C, Trifiro M and LeBlanc A , Estrogen and androgen protect against intracellular amyloid beta toxicity , J Neurosci , 2004 , 24: 5315-5321. Y, Zhang Y McLauglin R, Vereker E, Goodyer C and LeBlanc A , electively cytotoxicty of intracellular amyloid beta peptide1-42 through p53 and Bax in cultured primary human neurons , J Cell Biol , 2002 , 156: 519-529. Y, Zhang Y Goodyer C and LeBlanc A , Selective and protracted apoptosis in human primary neurons microinjected with active caspase-3, -6, -7 and –8 , J Neurosci , 2000 , 20: 8384-8389.

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